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Recent Replies
Comment: featureCounts results: low rate of 'Successfully assigned alignments'
by
GenoMax
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> I run a blast with the sequences and most of them belongs to globin.
Globin reads shown in your FastQC report are not going to add up to…
Comment: featureCounts results: low rate of 'Successfully assigned alignments'
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kvn95ss
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Almost all your samples have the over represented sequence, you can blast the top 10 recurring sequences and see what it could be.
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Linked read kits have not been offered by 10x genomics from last 3-4 years so that is not an option.
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Try taking out the additional header lines from the combined GTF file. `NC_005089.1` lines should be immediately followed by `NC_001846.1` …
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Nitin Narwade
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Hmmmmm, okay.
In this case I could think of another workaround, instead of drawing line/marks, you can add another modality to your plot, …
Comment: featureCounts results: low rate of 'Successfully assigned alignments'
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Hamtaro
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I added the fastQC. What do you think of the possible cause I have proposed?
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This isn't a bioinformatics question. What do you mean by "install all the dependencies"? Did you read the error message? It's telling you …
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Have you read the error message? it tells you to try installing the Magick++ library:
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Try installing:
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The first half of this vector should be your minimal fluxes per reaction and the second half your maximal fluxes per reaction. Thus this ve…
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Currently I am experiencing the same problem. Have you solved this?
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Good Information Sir
Comment: Viral genes not showing up in combined mouse+virus alignment
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Hello I was curious to follow up and wonder if anyone knew why the viral genes are not showing up in the count matrix? I can't seem to solv…
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CTLong
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That makes sense. Thanks for the reminder.
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Then you probably are best served by talking with your local sys admins to see where the issue may be.
Comment: Discrepancy in total number of bases in trimmed read1 and read2 files after BBDu
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> even if tpe and tbo are trailing after adapter and quality trimming
Priority/order of operations in coded in the program. Brian will be …
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