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Tutorial:
CITEseq tutorial
1 day ago by
Ming Tommy Tang
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12 days ago by
rohitsatyam102
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26 days ago by
Coremine Medical
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Answer: How to avoid missannotated GO terms?
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Istvan Albert
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The problem most likely is with the blast alignment.
BLAST is a local aligner, getting a hit to another sequence does not mean that the…
Comment: Read count vs Depth
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Ram
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Do not use spreadsheet software to view plain text files. Use a plain text utility such as BBEdit/TextWrangler/NotePad++/gedit or simple co…
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Ram
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Please stop asking basic R/programming questions and use Google to learn the underlying concepts. Your best bet to create customized plots …
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Ram
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What output files were you expecting? Was nothing printed on the screen at all?
Comment: Consensus peaksets in DiffBind - conceptual questinos
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Rory Stark
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The question of defaults for the software is often a tricky one.
I do think the mutliple-replicate method is a good idea in many cases, w…
Answer: How to query 1000 genomes project VCF files for specific regions without downloa
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Pierre Lindenbaum
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bcftools works with remote indexed VCF
bcftools view "http://ftp.1000genomes.ebi.ac.uk/vol1/ftp/data_collections/1000G_2504_high_cov…
Comment: Consensus peaksets in DiffBind - conceptual questinos
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Sam
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My question was not how to do it - but conceptually, should not this be the default way to do an analysis, unless there is a good reason *n…
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bk11
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Instead of `setdiff(list1$Sample_ID, list2$Sample_ID)` do this-
`setdiff(list1, list2)`
Answer: Consensus peaksets in DiffBind - conceptual questinos
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Rory Stark
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What you describe - deriving a consensus set comprising the union of peaks called in at least two replicates of any sample group - is exact…
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Matthias Zepper
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Then you use the resulting IDs to subset your original data frame(s).
Comment: convert dataframe R
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fracarb8
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You need to pass a column, so `id.vars = "rows"` should work.
Comment: convert dataframe R
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satva72
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Thanks. there is this error:
"Error in melt.data.table(data, id.vars = rownames(data)) :
One or more values in 'id.vars' is invalid."
T…
Answer: How to query 1000 genomes project VCF files for specific regions without downloa
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We have the 3202 samples from the 1kg DRAGEN called high coverge gVCFs on TileDB. If you sign up there we can get you some credits and assi…
Answer: convert dataframe R
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fracarb8
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More a programming question than bioinfo, but using `data.table` you can
library(data.table)
df <-as.data.frame(matrix(runif(100,…
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