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Recent Replies
Answer: Align miRNA library (small RNA-seq) without trimming
by
Ming Tommy Tang
★
2.9k
bowtie 1 is good for short read < 50bp, how long is your read? bowtie2 is better for reads > 50 bp
Comment: Differences in RNAseq Variant Calling and Allele Specific Expression
by
afei
•
0
Hi!
I'm also confused by this. And the site (https://gatkforums.broadinstitute.org/gatk/categories/gatk-support-forum) is closed. Have you …
Comment: Variant caller reports a homozygous variant genotype, but more reads are associa
by
rebeliscu
▴
50
Indeed, I thought the quality might be a factor. Still, given the information, it seems odd to me that that call was made.
Thanks for you…
Answer: How can I use bcftools mpileup or an alternative to find ALL variants without an
by
cfos4698
▴
730
To generate a VCF file one would normally pipe the input of an `mpileup` command into an actual `call` command. For example:
```
bcftools …
Answer: PTM-aware protein folding / docking
by
Mensur Dlakic
★
23k
Your assumption is correct - AlphaFold pTM has nothing to do with post-translational modifications. See [**here**][1].
Not to discourage…
Answer: Admixture cv error
by
Declan
•
0
I had this issue today and was able to resolve it by changing the random seed Admixture uses by adding `-s time` (which sets the random see…
Answer: Total No of Genes of GENCODE Release 43
by
ahmad
•
0
The following is the correct code
gtf_43<-rtracklayer::import('gencode.v43.primary_assembly.annotation.gtf')
dtgtf_43<-data.frame(gtf_4…
Comment: Total No of Genes of GENCODE Release 43
by
ahmad
•
0
Hello,
thanks for replying, by mistake I paste the wrong code. the following is the corrected
gtf_43<-rtracklayer::import('gencode.v43…
Comment: Kallisto bustools for scRNA-seq
by
dsull
★
4.0k
I have no idea what that is besides a bunch of jumbled sequences. You'll need look up the structure of the reads / library from wherever yo…
Comment: Aberrant splicing in bulk RNAseq
by
dsull
★
4.0k
First thing: kallisto cannot interface with UMI-tools (kallisto doesn't use read headers and therefore you're stuck with "the kallisto way"…
Comment: Using Copy Number Alterations detected in other studies for the same tumor cell
by
lethalfang
▴
90
There is some somatic SV study on these HCC1395: https://doi.org/10.1186/s13059-022-02816-6.
There is also a CNA preprint from years ago b…
Comment: Somatic truth set
by
lethalfang
▴
90
Just in case things are hard to find, for the WGS/WES, these are the data (what the file names mean: https://sites.google.com/view/seqc2/ho…
Comment: How to get subset of a Seurat object based on metadata?
by
jv
★
1.0k
Hmm, not sure why `subset` doesn't match to '0:CD8 T cell' but maybe it's the ":"?
An alternative option is the following:
```
idx <- whi…
Comment: GO enrichment analysis
by
Jeremy
▴
780
g:Profiler has an Arabidopsis option:
[g:Profiler][1]
[1]: https://biit.cs.ut.ee/gprofiler/gost
Answer: Any tips in landing a bioinformatic job?
by
Carambakaracho
★
3.2k
The first job is a nightmare and you will need some luck. I understand you're looking for an industry offering. Unfortunately I've worked o…
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