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Tutorial:
Create de novo repeat library
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Answer: Unmapped Reads in Kallisto
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dsull
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Running kallisto bus with the -n option will record the read numbers of the reads that have been successfully mapped in the output BUS file…
Comment: Automate the Splitting of a VCF File by Sample (bcftools)
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cfos4698
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Just a comment re: your bash example reading from a file. Have you tried `while` instead of 'with'?
```
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bcftools…
Comment: Split plink files by a number of SNPs
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Out of curiosity, why would someone ask how to do something if they already knew how to do it or which tools to use?
Also, the OP is not a…
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Zhenyu Zhang
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no.
The best you can do is to draw conclusion using one dataset, and validate your conclusion using other datasets.
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Thank, Seniors.. I figured out the issue of my integration as I did not state the ``` reduction= "harmony" ```
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> I merged two datasets in plink1.9. It worked, but I did get the error
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Comment: How to subset large BAM files specifically/ extract specific subsets?
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ella
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It worked now, after re-mapping with short-headed FASTA! :) Thanks a lot again for your time.
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Dear LChart,
thanks a million for your detailed response and explanations !! Some quick updated comments (hopefully not disturb you furth…
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Just because the articles and tutorials I read, they usually clearly separate which I worry I did something wrong.
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> do you think the one or other option (using fasta with shortened
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> However, in my analysis, clusters of each cell types are not clearly
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ella
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I think I even used `reformat.sh` earlier today, but don´t remember with what arguments. I now tried what you suggested, but got an error:
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Are you doing the clustering in UMAP space? If so, avoid distance-based clustering because distances are only meaningful locally, i.e dista…
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