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318 results • Page
3 of 7
Sort: Rank
Rank
Views
Votes
Replies
24
votes
4
replies
7.1k
views
Tutorial:
Using EnrichedHeatmap for visualization of NGS experiments
heatmap
chip-seq
atac-seq
EnrichedHeatmap
updated 8 months ago by
Ram
41k • written 4.3 years ago by
ATpoint
78k
15
votes
4
replies
4.4k
views
Tutorial:
RNASeq tutorial for gene differential expression analysis
Transcriptomics
bioconductor
RNA-Seq
R
updated 8 months ago by
Ram
41k • written 3.3 years ago by
Thind amarinder
▴ 340
26
votes
0
replies
2.3k
views
Tutorial:
Resources / databases for clinical interpretation of cancer variants
interpretation
cancer
annotation
variant
updated 8 months ago by
Ram
41k • written 4.1 years ago by
Malachi Griffith
19k
10
votes
1
reply
2.2k
views
Tutorial:
introduce a tool for fast identification of SARS-CoV-2 and other microbes from sequencing data
SARS-CoV-2
fastv
COVID-19
updated 8 months ago by
Ram
41k • written 3.7 years ago by
chen
★ 2.5k
60
votes
52
replies
42k
views
23 follow
Tutorial:
Creating Interactive Volcano Plots with R and Plot.ly
plotly
R
volcano-plot
updated 8 months ago by
Ram
41k • written 7.2 years ago by
steve
★ 3.4k
13
votes
7
replies
5.6k
views
Tutorial:
STARsolo config for 10x Chromium v1, v2, v3
Chromium
10x
STARsolo
STAR
updated 8 months ago by
Ram
41k • written 3.2 years ago by
Kevin Blighe
86k
18
votes
22
replies
8.4k
views
10 follow
Tutorial:
Create de novo repeat library
de-novo
repeat
annotation
updated 10 weeks ago by
evoecogen
▴ 20 • written 4.0 years ago by
Juke34
8.4k
0
votes
0
replies
1.0k
views
Tutorial:
BrainArray CDF, annotation, etc
db
brainarray
cdf
updated 8 months ago by
Ram
41k • written 3.2 years ago by
Kevin Blighe
86k
12
votes
0
replies
2.5k
views
Tutorial:
Long versus wide data for plotting genomics data in R.
ggplot2
tidyverse
data.table
R
updated 8 months ago by
Ram
41k • written 3.3 years ago by
rpolicastro
12k
76
votes
70
replies
18k
views
18 follow
Tutorial:
Clustering of DAVID gene enrichment results from gene expression studies
enrichment
david
updated 8 months ago by
Ram
41k • written 5.8 years ago by
Kevin Blighe
86k
1
vote
1
reply
1.3k
views
Tutorial:
Downloading GnuWin and using to convert txt to bed file for windows users
bed
bed2txt
sequencing
txt
updated 8 months ago by
Ram
41k • written 3.3 years ago by
opheevallee
• 0
4
votes
3
replies
4.4k
views
Tutorial:
How to get full taxonomy classification using taxize and myTAI
taxonomy
R
updated 8 months ago by
Ram
41k • written 5.6 years ago by
Daniel
★ 3.9k
4
votes
1
reply
1.3k
views
Tutorial:
Real World BEDtools. Practical Examples for Variant Filtering and Gene Annotations
BED
BEDtools
updated 8 months ago by
Ram
41k • written 3.3 years ago by
sdsubaru33
▴ 40
3
votes
0
replies
3.4k
views
Tutorial:
Sorting GFF/GTF files
gtf
gff
updated 8 months ago by
Ram
41k • written 3.4 years ago by
Juke34
8.4k
20
votes
6
replies
5.3k
views
Tutorial:
Open Targets and programmatic access
open-targets
disease
API
drug
updated 8 months ago by
Ram
41k • written 7.2 years ago by
Denise CS
★ 5.2k
2
votes
2
replies
1.9k
views
Tutorial:
Human mitochondrial DNA annotation in SnpEff
chrMT
SNP
snpEff
updated 8 months ago by
Ram
41k • written 3.4 years ago by
Arup Ghosh
3.2k
12
votes
5
replies
2.2k
views
Tutorial:
FASTA file split
fasta
updated 8 months ago by
Ram
41k • written 3.5 years ago by
Juke34
8.4k
0
votes
0
replies
1.6k
views
Tutorial:
VIDEO: Installing BLAST Databases on Your Own Computer
BLAST-databases
Linux
sequence
MacOSX
updated 8 months ago by
Ram
41k • written 3.5 years ago by
brian.fristensky
▴ 450
0
votes
0
replies
938
views
Tutorial:
VIDEO: BLAST Searches Through a Data Science Lens
Covid19
Data-Science
sequence
BLAST
updated 8 months ago by
Ram
41k • written 3.5 years ago by
brian.fristensky
▴ 450
2
votes
1
reply
1.5k
views
Tutorial:
A very simple way to detect viruses and microorganisms from mNGS FASTQ data using fastv
SARS-CoV-2
fastv
COVID-19
viruses
updated 8 months ago by
Ram
41k • written 3.5 years ago by
chen
★ 2.5k
72
votes
26
replies
27k
views
15 follow
Tutorial:
How To Separate Illumina Based Strand Specific Rna-Seq Alignments By Strand
RNA-seq
updated 9 months ago by
Ram
41k • written 9.8 years ago by
Istvan Albert
99k
59
votes
43
replies
20k
views
18 follow
Tutorial:
Polish PacBio assembly with latest PacBio tools : an affordable solution for everyone
pbalign
pacbio
arrow
smrt-limk
updated 8 months ago by
Ram
41k • written 6.2 years ago by
Rox
★ 1.4k
20
votes
7
replies
6.1k
views
Tutorial:
Showing up and down regulated GO and pathway using enrichR and ggplot2
ggplot2
RNA-Seq
R
updated 8 months ago by
Ram
41k • written 5.1 years ago by
ahmad mousavi
▴ 770
4
votes
0
replies
1.7k
views
Tutorial:
NIH CDE browser for TCGA clinical data CDE codes
nih
tcga
metadata
cde
updated 8 months ago by
Ram
41k • written 3.7 years ago by
Kevin Blighe
86k
10
votes
4
replies
11k
views
Tutorial:
Using the MinION MinKNOW software on Ubuntu 1604
long-reads
oxford-nanopore
minion
updated 8 months ago by
Ram
41k • written 4.4 years ago by
colindaven
5.7k
0
votes
0
replies
1.2k
views
Tutorial:
Training GeneMark-EP+ ab-initio tool
abinitio
training
genemark
annotation
updated 8 months ago by
Ram
41k • written 3.8 years ago by
Juke34
8.4k
2
votes
1
reply
2.9k
views
Tutorial:
How to extract and filter SNP data from the genotyping-by-sequencing (GBS) data in vcf format using bcftools
bcftools
SNP-Filtering
VCF
updated 8 months ago by
Ram
41k • written 3.8 years ago by
Asad Prodhan
▴ 10
5
votes
0
replies
3.1k
views
Tutorial:
How to run CWL with Nextflow
cwl
nextflow
updated 8 months ago by
Ram
41k • written 3.8 years ago by
steve
★ 3.4k
5
votes
2
replies
2.2k
views
Tutorial:
Using Bedtools Closest (closestBed) to make distance histograms of protein binding frequency near all types of pre-RNA junctions (e.g. exon-intron an…
preRNA
closestBed
bedtools-closest
bedtools
updated 8 months ago by
Ram
41k • written 4.8 years ago by
linuxborg2
▴ 10
50
votes
3
replies
4.5k
views
Tutorial:
[[ Please read before posting a question ::: How To Ask A Good Question ]]
how-to
updated 8 months ago by
Ram
41k • written 4.9 years ago by
ATpoint
78k
21
votes
9
replies
6.3k
views
8 follow
Tutorial:
Interactive Perl Learning
perl
updated 9 months ago by
Ram
41k • written 10.5 years ago by
Medhat
9.7k
282
votes
39
replies
177k
views
29 follow
Tutorial:
How to download raw sequence data from GEO/SRA
fastq
SRA
bam
GEO
updated 8 months ago by
Ram
41k • written 9.3 years ago by
Obi Griffith
20k
64
votes
26
replies
13k
views
7 follow
Tutorial:
Parallel processing in R
biocparallel
mclapply
foreach
R
updated 8 months ago by
Ram
41k • written 6.2 years ago by
Kevin Blighe
86k
34
votes
15
replies
6.0k
views
6 follow
Tutorial:
How to do data cleaning for VCF genetic file
vcf
updated 8 months ago by
Ram
41k • written 4.5 years ago by
Shicheng Guo
★ 9.3k
20
votes
4
replies
2.5k
views
Tutorial:
Bash One-liners collection on Github
bash
linux
shell
updated 8 months ago by
Ram
41k • written 4.6 years ago by
nim.1111.ou
▴ 180
14
votes
7
replies
6.1k
views
Tutorial:
Visualization of ChIP-Seq peak overlaps using HOMER mergePeaks and UpSetR
ChIP-Seq
peaks
R
HOMER
updated 8 months ago by
Ram
41k • written 7.5 years ago by
steve
★ 3.4k
2
votes
0
replies
4.4k
views
Tutorial:
Calculation of TSS Scores for ATAC-seq Data
next-gen-sequencing
updated 8 months ago by
Ram
41k • written 4.4 years ago by
Sael
▴ 20
0
votes
0
replies
1.0k
views
Tutorial:
Apply ALoFT to identify loss-of-function somatic or germline variants
ALoFT
updated 8 months ago by
Ram
41k • written 4.5 years ago by
Shicheng Guo
★ 9.3k
16
votes
11
replies
14k
views
7 follow
Tutorial:
retrieve full TCGA datasets from cBioportal with R
TCGA
RNA-seq
data-retrieval
cBioportal
R
updated 8 months ago by
Ram
41k • written 7.1 years ago by
TriS
★ 4.7k
10
votes
5
replies
21k
views
Tutorial:
Y-DNA and mtDNA Haplogroup Predictor
haplogroup
updated 8 months ago by
Ram
41k • written 5.4 years ago by
Shicheng Guo
★ 9.3k
34
votes
15
replies
19k
views
12 follow
Tutorial:
TCGA UUIDS to TCGA barcode (SampleID) in R
next-gen
GDC
R
TCGA
updated 8 months ago by
Ram
41k • written 7.2 years ago by
martinguerrerog89
▴ 310
6
votes
3
replies
4.4k
views
Tutorial:
Principles and Workflow of 16S/18S/ITS Amplicon Sequencing
alignment
sequencing
RNA-seq
gene
updated 8 months ago by
Ram
41k • written 5.1 years ago by
contact
▴ 40
0
votes
0
replies
1.0k
views
Tutorial:
Introduction to Gen3 Webinar
gen3
open-source
data-commons
big-data
updated 8 months ago by
Ram
41k • written 4.8 years ago by
Danielle
▴ 310
0
votes
1
reply
1.3k
views
Tutorial:
SFA (Stochastic Frontier Analysis) Frontier 4.1
r
spss
updated 8 months ago by
Ram
41k • written 5.8 years ago by
helgasaraswati
▴ 10
4
votes
2
replies
3.5k
views
Tutorial:
Introduce OpenGene: an open source library for processing NGS data
NGS
sequencing
Julia
OpenGene
updated 8 months ago by
Ram
41k • written 7.8 years ago by
chen
★ 2.5k
28
votes
4
replies
4.1k
views
Tutorial:
Modern Statistics for Modern Biology
statistics
updated 8 months ago by
Ram
41k • written 4.9 years ago by
Jean-Karim Heriche
26k
61
votes
6
replies
9.3k
views
Tutorial:
Minimum Standards For Bioinformatics Command Line Tools
command-line
updated 9 months ago by
Ram
41k • written 10.3 years ago by
Medhat
9.7k
4
votes
0
replies
945
views
Tutorial:
Finding individual motif occurrences with FIMO from the MEME suite
motif
meme
jaspar
fimo
updated 8 months ago by
Ram
41k • written 5.0 years ago by
ATpoint
78k
5
votes
2
replies
2.2k
views
Tutorial:
NCBI submissions: SRA and TSA
NCBI
TSA
SRA
RNA-Seq
updated 8 months ago by
Ram
41k • written 5.0 years ago by
saraoppenheim
▴ 50
6
votes
0
replies
1.5k
views
Tutorial:
NCBI dbVar releases updated human reference structural variation (SV) data files and tutorials
dbvar
structural-variation
updated 8 months ago by
Ram
41k • written 5.0 years ago by
lonphan
▴ 70
318 results • Page
3 of 7
Recent Votes
Comment: Problematic fastq files...How can we trust them?
Answer: GO categorization
Comment: Using metagenome assembly and binning to identify and mitigate contamination in
Answer: How to obtain data on the coordinates of the Exon region from UCSC
Answer: How to obtain data on the coordinates of the Exon region from UCSC
Answer: How to obtain data on the coordinates of the Exon region from UCSC
Answer: An incomprehensible error with R package gggenes
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Comment: Yes .. BBMap can do that! - Part III clumpify (mark (and dedupe) duplicates with
by
GenoMax
136k
Yes that should be true. I will let https://www.biostars.org/u/14684/ confirm.
Comment: From TPM to raw counts
by
Gama313
▴ 120
Thanks for the answe and the linkI used bioinfokit tpm formula to calculate tpm from bulk which is the same formula given in your link: A= …
Comment: From TPM to raw counts
by
Gama313
▴ 120
Thanks Brian for the suggestion. However, I did the whole process, from bulk counts generation, to data transformation and scrna deconvolut…
Answer: Are 10x cellranger-arc ATAC bam files deduplicated?
by
swbarnes2
13k
My experience with regular 10x bam files is that duplicates are not removed, but they are flagged as duplicates. So take a look at the …
Comment: Can I use TCGA-LUAD RNAseq count that had already normalized by RSEM in Limma-vo
by
fluke
• 0
Thanks a lot for your answer, I’m confused between RSEM expected count and RSEM normalized count. I’m working on this dataset from UCSC ht…
Answer: From TPM to raw counts
by
Istvan Albert
99k
In principle, the TPM formula can be reverted, see the timeless post * [What the FPKM? A review of RNA-Seq expression units][1] In p…
Comment: Low coverage whole genome sequencing reveal excess heterozygosity for multiple S
by
beausoleilmo
▴ 560
You're right, I wasn't explaining the problem clearly. Thanks for the directions! - The depth; coverage ~3.00X ± 2.50 SD - The sequen…
Comment: Using metagenome assembly and binning to identify and mitigate contamination in
by
Mensur Dlakic
★ 25k
All good points, especially about multiple copies of single-copy genes. I am doing error-correction in my assemblies, but was making an edu…
Comment: Can I use TCGA-LUAD RNAseq count that had already normalized by RSEM in Limma-vo
by
CTLong
▴ 20
Yes, normalized RSEM counts from TCGA can be used as input for Limma Voom. Please check this post https://support.bioconductor.org/p/63981/…
Comment: How to obtain data on the coordinates of the Exon region from UCSC
by
ayasu
• 0
Sorry for the delay in expressing my thanks. I found the advice to look at the information from MySQL very useful. I will also refer to t…
Comment: From TPM to raw counts
by
Brian Bushnell
19k
I'm posting this as a comment instead of an answer specifically because it's just what I would do and I don't know if it's the best approac…
Comment: Using metagenome assembly and binning to identify and mitigate contamination in
by
Brian Bushnell
19k
In most cases error-correction should take care of error-spawned fake minor alleles, though... > If you want to convince yourself of this,…
Answer: Using metagenome assembly and binning to identify and mitigate contamination in
by
Mensur Dlakic
★ 25k
It is a valid question, and I particularly like when posters err on the side of providing more than less detail. Metagenomic binning can be…
Answer: Low coverage whole genome sequencing reveal excess heterozygosity for multiple S
by
Brian Bushnell
19k
I don't understand your plot. Perhaps a legend would help? I also don't know what you mean by "genotype frequency"; is that the ratio of …
Comment: Does GNOMAD use all LOFTEE LoF filters?
by
AMARU
• 0
Can you post the commands you used? I am having some issues running that plugin on vep v110. It appears in fields but it doesn't annotat…
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