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Answer: Pediococcus acidilactici sequence analysis
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Answer: Assume "yes" as the answer to any prompts with bioconda
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Juke34
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From my experience using `biocontainers` base container no need to set `yes` installation is done without any command prompt, otherwise as …
Comment: SLURM submission problem: Indexing the Reference Genome is not done in STAR
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You're allocating 4 compute nodes (`#SBATCH -N 4`), but STAR can't actually make use of more than 1 compute node. You can increase the num…
Answer: Assume "yes" as the answer to any prompts with bioconda
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```conda install -y bioconda::packageX conda-forge::packageY``` should do what you're looking for.
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Hi GenoMax, Thank you for your advice. I sent an email to them and have already opened an issue on Github. Let see if there is any other so…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
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me
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You can't. The data does not exist. UniProt only annotates where a lysine is know to be acetylated. There is no way that UniProt can know w…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
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Jacky
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Yes, that's what I meant.
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
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Jacky
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I'm sorry, I didn't explain it clearly. Below are my operational steps: I enter 'Triticum aestivum' in the search box and perform a search.…
Comment: Indexing the Reference Genome is not done in STAR
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I add these parameters; #SBATCH -N 4 #SBATCH --tasks=1 #SBATCH -c 4 #SBATCH --time=24:00:00 But it keeps giving error
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rrehimi
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Thanks! Could you please explain the DEseq2 method using Galaxy step by step? Input files?
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Is is this one - KOREF ? There might be multiple versions. https://www.ncbi.nlm.nih.gov/bioproject/PRJNA294231/ If it is, then take that…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
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me
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The question is not quite clear? are you asking for wheat proteins in UniProtKB which contain a lysine amino-acid, where those lysine are *…
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You should provide row count (without normalization) for doing DEG analysis by relevant tools such as, edgeR or DEseq.
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Thank you. I have another question. Is there a way to get the gene list under "setSize" from the output that you gave as an example.
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Hi, i have added the fastqc plots (untrimmed and trimmed). The sample was from anaerobes enriched from wastewater treatment plant, and this…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
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Pierre Lindenbaum
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> lysine non-acetylated sequences what does it look like in uniprot ? give us an example with a uniprot identifier.
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