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Showing top results for tag: CRISPR • reset
2
votes
2
replies
165
views
Tools for CRISPR/CAS9
CRISPR tool CAS9
updated 2 days ago by 49k • written 2 days ago by • 0
0
votes
2
replies
159
views
Recommendation for computational tools to detect gene editing induced INDELs from amplicon NGS data
CRISPR Gene-editing CRISPResso2 Amplicon-sequencing ZFN
updated 3 days ago by ▴ 480 • written 3 days ago by • 0
7
votes
5
replies
455
views
Analysis of shRNA/CRISPR screens in 2021
crispr shrna
updated 27 days ago by ★ 2.3k • written 9 weeks ago by 54k
0
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0
replies
148
views
Indel frequency analysis from CRISPR modification using sequencing data.
CRISPR Indel Cas9 frequency site cut
9 weeks ago by ms3389 • 0
0
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0
replies
146
views
CRISPR cut site annotation of gene feature and consequence
off-target crispr annotation gff knockout
10 weeks ago by James Sacco • 0
0
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0
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135
views
Job: Postdoc in Metabolic Engineering
CRISPR FACS HPLC biology molecular
11 weeks ago by info ▴ 70
0
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0
replies
86
views
Mitochondrial targeting sequence
mts crispr geneediting gene Sequence
3 months ago by chiti.agarwal • 0
0
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0
replies
120
views
Analysis of CRISPR screening with very strong positive selection
MAGeCK CRISPR
3 months ago by di4mond • 0
2
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3
replies
278
views
How to Align multiple short reads (NGS data) to a short (~200bp) reference?
genomics NGS CRISPR genotyping
updated 3 months ago by 34k • written 4 months ago by ▴ 10
1
vote
2
replies
207
views
need help
microalgae bioremediation CRISPR Cas9
updated 4 months ago by 107k • written 4 months ago by • 0
0
votes
1
reply
245
views
gRNA concatamer - will it work ?
gRNA CRISPR PAM
updated 5 months ago by 54k • written 5 months ago by ▴ 10
0
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0
replies
272
views
Job: Research assistant for yeast natural products project at DTU Biosustain
yeast preps DNA DTU Biosustain CRISPR
5 months ago by info ▴ 70
0
votes
0
replies
270
views
Job: Job: CRISPR Microbiome Bioinformatician
CRISPR microbiome job bioinformatician denmark Job
6 months ago by evanderhelm • 0
1
vote
3
replies
273
views
How do you design primers with sequencing adapters to amplify CRISPR-edited regions?
primer-design crispr primer3
updated 6 months ago by • 0 • written 6 months ago by ▴ 10
1
vote
3
replies
1.1k
views
What tools do you use to find the potential off-target sites when designing Cas9/Cas12 RNA-guided endonucleases?
CRISPR off-target alignment
6 months ago by genomics0101 ▴ 10
0
votes
0
replies
446
views
Human GeCKO library validation
CRISPR Library sgRNA GeCKO v2
9 months ago by mansisharma.iisc • 0
0
votes
0
replies
657
views
Job: Research assistant for Biotech Project at the Novo Nordisk Foundation Center for Biosustainability (CfB), Technical University of Denmark
CRISPR molecular biology DNA Job
10 months ago by info ▴ 70
0
votes
0
replies
669
views
Job: Staff Scientist, Ph.D., or Postdoctoral Position in Bioinformatics
RNA-Seq ChIP-Seq Cancer Epigenetics CRISPR Job
10 months ago by Gjain 5.6k
3
votes
5
replies
551
views
CRISPR/Cas9 screen analysis: reads R1 and R2 mixed
crispr screen reads illumina sequencing
14 months ago by Swimming bird ▴ 20
0
votes
2
replies
1.3k
views
How to retrieve all hmm from TIGRFAM for Cas (CRISPR-associated) proteins?
hmm Cas CRISPR
updated 18 months ago by 20 • written 4.5 years ago by ▴ 180
1
vote
5
replies
433
views
RNAseq Design Help
RNA-Seq DESeq2 R crispr cas9
18 months ago by tropeedward • 0
1
vote
0
replies
554
views
Job: PhD studentship in CRISPR bioinformatics and cancer genomics
sequencing next-gen CRISPR Cancer LncRNA Job
18 months ago by rory.johnson ▴ 20
0
votes
0
replies
301
views
about the design of guideRNA for CRISPRko/ CRISPRi/ CRISPRa
CRISPR
19 months ago by Bogdan ★ 1.1k
2
votes
7
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435
views
How to identify 100ish deletion from NGS data
CRISPR targeted sequencing
20 months ago by haewon • 0
1
vote
6
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896
views
Extract gRNA sequence using cutadapt
cutadapt trimming crispr sequencing
22 months ago by Swimming bird ▴ 20
0
votes
0
replies
411
views
News: Webinar: Single Cell Multi-omics Data Analysis in Partek Flow - Visualization of CRISPR Data
single cell bioinformatics CRISPR News
22 months ago by kgosche ▴ 30
9
votes
7
replies
1.8k
views
7 follow
Fix FoxG1 deletion in my son using CRISPR
FoxG1 CRISPR cure
updated 24 months ago by 20 • written 5.5 years ago by ▴ 10
0
votes
4
replies
559
views
GUIDEseq data links dead in github
GUIDEseq github Cas9 CRISPR guideseq
2.0 years ago by joe ▴ 230
1
vote
0
replies
397
views
CRISPR off-target validation with GUIDEseq
Cas9 GUIDEseq CIRCLEseq CRISPR
2.1 years ago by joe ▴ 230
0
votes
0
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419
views
News: Interested in learning more about CRISPR Genome Editing?
CRISPR GenomeEditing News
2.1 years ago by carlopecoraro2 ★ 2.0k
2
votes
0
replies
471
views
News: Course: Approaches and Computational tools for CRISPR Genome editing
CRISPR GenomeEditing News
2.3 years ago by carlopecoraro2 ★ 2.0k
2
votes
1
reply
3.3k
views
How to interpret CERES gene scores for genome wide CRISPR-Cas9 knockout screens?
CRISPR
2.3 years ago by patelk26 ▴ 250
0
votes
0
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645
views
News: CRISPR Genome Editing
genome CRISPR News
2.5 years ago by carlopecoraro2 ★ 2.0k
1
vote
0
replies
985
views
Job: Global Bioinformatics Manager - Horizon Discovery, Lafayette Colorado
RNA-Seq next-gen CRISPR gene editing siRNA Job
2.5 years ago by shawn.mek ▴ 10
0
votes
2
replies
658
views
What is the best way to costract a Crispr library
crispr library sgRNA
2.7 years ago by Assa Yeroslaviz ★ 1.6k
9
votes
3
replies
2.1k
views
Install CRISPRCasFinder under Ubuntu without having administrator rights
install crispr linux
updated 2.8 years ago by 46k • written 2.8 years ago by ▴ 80
0
votes
3
replies
2.6k
views
RIGER extension in GENE-E
sequencing next-gen crispr Gene Ranking
updated 3.0 years ago by ▴ 10 • written 4.0 years ago by • 0
0
votes
1
reply
906
views
Looking CRISPR-Cas9 sequencing Data
sequencing crispr ngs
3.2 years ago by bi_Scholar • 0
0
votes
1
reply
708
views
Analyzing CRISPR and RNAi genome wide screens
CRISPR RNAi meta analysis
3.3 years ago by spacemorrissey ▴ 260
1
vote
4
replies
1.1k
views
Newbie Question: How to find overlap of multiple cellline datas
ngs overlap CRISPR
updated 3.3 years ago by 20 • written 4.2 years ago by ▴ 50
4
votes
9
replies
1.1k
views
Genome-wide CRISPR/Cas9 and gene expression?
crispr cas9 microarray gene expression
updated 3.3 years ago by 11k • written 3.4 years ago by ▴ 170
1
vote
1
reply
965
views
Job: DIRECTOR of COMPUTATIONAL BIOLOGY, Cedilla Therapeutics, Cambridge, MA, USA
CRISPR Oncology High Throughput Sequencing Job
updated 3.4 years ago by 107k • written 3.4 years ago by ▴ 10
0
votes
0
replies
980
views
Job: Post-doc positions on non-coding RNAs in neurological diseases at RIKEN Yokohama Japan
single cell CRISPR lncRNA GWAS Job
3.4 years ago by chungchau.hon ▴ 10
0
votes
0
replies
645
views
How to analyse data from genetic screening followed by NGS ?
screening gene-trapping gRNA crispr DE-analysis
3.6 years ago by Assa Yeroslaviz ★ 1.6k
1
vote
1
reply
917
views
crispr mageck analysis ,Sam library
genome Crispr library Sam
updated 3.8 years ago by 138k • written 3.8 years ago by • 0
2
votes
0
replies
1.4k
views
CRISPR screening analysis using mageck
mageck crispr screening reads
4.1 years ago by Folder40g ▴ 160
12
votes
4
replies
1.2k
views
Forum: Update: CRISPR off-target effects causing unexpected mutations - good science or media hype?
CRISPR off target Forum
4.3 years ago by YaGalbi ★ 1.5k
1
vote
5
replies
1.1k
views
How to find which residues in a protein which are targetable by CRISPR
genome gene crispr cas9
updated 4.4 years ago by ▴ 570 • written 4.4 years ago by ▴ 10
0
votes
0
replies
2.0k
views
Job: Computational Postdoc positions available Harvard Medical School/Massachusetts General Hospital
MACHINE LEARNING SINGLE CELL EPIGENOMICS CRISPR Job
4.5 years ago by lucapinello • 0
0
votes
3
replies
937
views
CRISPR repeat identification tool comparison
alignment blast crispr
updated 4.5 years ago by 33k • written 4.5 years ago by ▴ 70
50 results • Page 1 of 1
Recent Votes
Comment: Weird error from BWA and BOWTIE2
C: Sequence Length Distribution From A Fastq File
Answer: How can I extract upstream regions in fasta for all blastx hits locally performe
C: What Are The Most Common Stupid Mistakes In Bioinformatics?
Comment: Beginner problem: installed python3.7.12 but py_available() return FALSE
A: Automatic Outlier Detection for RNA-seq data
A: ATAC-seq sample normalization (quantil normalization)
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Teacher to swbarnes2 11k
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Recent Replies
Comment: Importing multiple datasets into one folder by Mensur Dlakic ★ 13k
Are you interested in moving all `.fastq` files from various folders into one? Because that can be done with a single command, but not sure…
Comment: Beginner problem: install python3.7.12 but py_available() still return FALSE by Ram 34k
Please do not delete the post after it has received feedback.
Comment: How can I know the BLAST percentage from kraken2 files ? by h.mon 33k
You can't. In no moment whatsoever Kraken2 performs an alignment. Morever, BLAST is BLAST, Kraken2 is Kraken2, they are not related and p…
Comment: Importing multiple datasets into one folder by GenoMax 107k
Galaxy specific questions are best asked on their dedicated support forum: https://help.galaxyproject.org/
Comment: circulating toumor DNA by GenoMax 107k
Since all cells shed [** free DNA**][1] that circulates (`cfDNA`) (including tumor cells) I don't know if there needs to be a separate data…
Comment: Automatic Outlier Detection for RNA-seq data by Kevin Blighe 77k
There is actually, well, a sort of citation off the top of my head. Please see the PLINK documentation (section '*Outlier detection diagnos…
Answer: Batch effects by swbarnes2 11k
It's always safest to get raw data and normalize it yourself. If one dataset is control, and the other is not, there is no magic way to …
Comment: GREP from multiFasta file and keep headers by Pierre Lindenbaum 138k
your command line with `grep` **implies** that each record in your fasta file takes only two line (?). One line for the header, and one lin…
Answer: In what way should cellranger count be performed , when there are multiple runs by swbarnes2 11k
If you did **one** library prep and ran that one library prep multiple times, it is probably easiest to combine the fastqs (make sure to gi…
Comment: Splice aware vs splice unaware aligners? by swbarnes2 11k
I think for time's sake a not-splice aware aligner isn't going to search horribly far away for the other half of a read, while a splice-awa…
Comment: Beginner problem: installed python3.7.12 but py_available() return FALSE by Sashu • 0
Anyway, thanks your reply!
Comment: Automatic Outlier Detection for RNA-seq data by simplitia ▴ 60
@kevin thank is a great answer. We are having the same issue with outliers. With the 2nd part using > 3 SD; do you have a reference for …
Comment: How to use MACS2 output for DESEQ2 differential analysis during ATAC-seq? How to by Papyrus ★ 1.3k
Take a look at the [DiffBind][1] R package (which internally uses `DESeq2`, or `edgeR`), the tutorial [vignette][2] is quite extensive. …
Comment: Beginner problem: installed python3.7.12 but py_available() return FALSE by Sashu • 0
I forget to tell that I have followed the error message but this problem still existed...
Comment: Beginner problem: installed python3.7.12 but py_available() return FALSE by jared.andrews07 ★ 11k
Follow the error message. Allow reticulate to handle the python environment itself.
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