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1
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3
replies
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How to check gene properties (eg GC, repetitiveness, average expression level)?
deseq2 kallisto RNA-seq
updated 14 months ago by 43k • written 17 months ago by ▴ 150
0
votes
10
replies
1.2k
views
Can I demultiplex run based on i5 sequence?
demultiplexing bcl2fastq
updated 17 months ago by 141k • written 17 months ago by ▴ 150
3
votes
13
replies
3.1k
views
How to choose parameters for kallisto single end mode?
kallisto RNA-seq
updated 11 months ago by 100k • written 18 months ago by ▴ 150
7
votes
3
replies
1.9k
views
Should I merge my technical replicates for bulk RNA seq?
RNA-seq DESeq2
updated 14 months ago by 43k • written 18 months ago by ▴ 150
4
votes
4
replies
989
views
Can I perform DE analysis with Seurat if I only have one sample per condition?
single RNA Seq cell seurat
updated 18 months ago by 3.9k • written 18 months ago by ▴ 150
0
votes
0
replies
359
views
How do I get estimated gene counts for a reference that contains GFP using Kallisto?
gene ensembl tximport kallisto
19 months ago by bioinfo ▴ 150
4
votes
4
replies
1.3k
views
How can I confirm that I installed bcl2fastq correctly?
bcl2fastq linux ubuntu
19 months ago by bioinfo ▴ 150
3
votes
2
replies
610
views
Why do I get a positive gene expression with qPCR for one my genes but in with when doing bulk RNA seq I get a count of 0?
gene qpcr kallisto seq expression rna
updated 20 months ago by ★ 5.8k • written 20 months ago by ▴ 150
1
vote
3
replies
768
views
How to filter .fa file by gene_biotype
rna-seq kallisto
updated 22 months ago by 43k • written 23 months ago by ▴ 150
0
votes
7
replies
1.2k
views
6 follow
How to create kallisto index that does not include ribosomal genes
enseml kallisto RNA-seq
updated 16 months ago by 43k • written 23 months ago by ▴ 150
0
votes
0
replies
370
views
Replicates in sc-RNA seq have different amount of cells-Can I still use them for the analysis?
Cell seurat Single
updated 23 months ago by 81k • written 23 months ago by ▴ 150
2
votes
4
replies
1.0k
views
Can I use the transcripts_to_genes.txt file from kallisto for bulk RNA seq data?
kallisto
updated 24 months ago by 43k • written 2.0 years ago by ▴ 150
2
votes
3
replies
1.1k
views
Ensembl version for mapping and annotating genes
rna-seq kallisto
updated 22 months ago by 43k • written 2.0 years ago by ▴ 150
6
votes
2
replies
582
views
Ensembl version
ensembl kallisto
updated 16 months ago by 43k • written 2.0 years ago by ▴ 150
2
votes
2
replies
2.3k
views
Cellranger command for RNA velocity
cellranger Cell velocity RNA Single
updated 2.2 years ago by 8.9k • written 2.2 years ago by ▴ 150
4
votes
2
replies
920
views
Estimated gene counts with tximport
RNA-seq ensembl tximport kallisto
updated 22 months ago by 43k • written 2.3 years ago by ▴ 150
3
votes
3
replies
1.3k
views
Will Kallisto show a decrease in the TPM value if I have knocked out 2 of the 8 exons from a gene?
RNA-seq
updated 22 months ago by 43k • written 2.4 years ago by ▴ 150
2
votes
2
replies
1.1k
views
How to demultiplex a single indexed library on a dual indexed flow cell?
Cell Single
updated 2.4 years ago by 141k • written 2.4 years ago by ▴ 150
68 results • Page 2 of 2
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