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1
vote
0
replies
513
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What is the difference between gseapy.prerank and gseapy.gsea?
gseapy gsea
20 months ago by bioinfo ▴ 150
3
votes
2
replies
645
views
Do I need to correct for sequencing depth in bulk RNA seq?
kallisto RNA-seq
updated 20 months ago by 44k • written 20 months ago by ▴ 150
0
votes
0
replies
589
views
How to adjust graph in scanpy?
scanpy single-cell RNA-seq
20 months ago by bioinfo ▴ 150
0
votes
0
replies
765
views
How to analyze scRNA seq data with time points (Seurat/Pseudotime)?
monocle3 scRNA-seq pseudotime Seurat R
20 months ago by bioinfo ▴ 150
2
votes
2
replies
1.3k
views
How to perform scRNA seq analysis?
single-cell pseudobulk seurat
21 months ago by bioinfo ▴ 150
3
votes
2
replies
884
views
Does the bootstrap in kallisto affect the BAM files?
RNAseq kallisto BAM
21 months ago by bioinfo ▴ 150
1
vote
4
replies
1.5k
views
Issues with chromosome file in kallisto
kallisto
updated 21 months ago by ★ 6.9k • written 21 months ago by ▴ 150
1
vote
2
replies
970
views
How to run UMAP on single cell results from 2 different runs?
single-cell seurat cellranger
updated 22 months ago by 4.3k • written 22 months ago by ▴ 150
1
vote
3
replies
859
views
How to check gene properties (eg GC, repetitiveness, average expression level)?
deseq2 kallisto RNA-seq
updated 20 months ago by 44k • written 22 months ago by ▴ 150
0
votes
10
replies
1.5k
views
Can I demultiplex run based on i5 sequence?
demultiplexing bcl2fastq
updated 22 months ago by 146k • written 22 months ago by ▴ 150
3
votes
13
replies
3.8k
views
How to choose parameters for kallisto single end mode?
kallisto RNA-seq
updated 16 months ago by 101k • written 23 months ago by ▴ 150
7
votes
3
replies
2.4k
views
Should I merge my technical replicates for bulk RNA seq?
RNA-seq DESeq2
updated 20 months ago by 44k • written 23 months ago by ▴ 150
4
votes
4
replies
1.2k
views
Can I perform DE analysis with Seurat if I only have one sample per condition?
single RNA Seq cell seurat
updated 23 months ago by 4.3k • written 23 months ago by ▴ 150
0
votes
0
replies
423
views
How do I get estimated gene counts for a reference that contains GFP using Kallisto?
gene ensembl tximport kallisto
2.0 years ago by bioinfo ▴ 150
4
votes
4
replies
1.5k
views
How can I confirm that I installed bcl2fastq correctly?
bcl2fastq linux ubuntu
2.1 years ago by bioinfo ▴ 150
3
votes
2
replies
718
views
Why do I get a positive gene expression with qPCR for one my genes but in with when doing bulk RNA seq I get a count of 0?
gene qpcr kallisto seq expression rna
updated 2.2 years ago by ★ 6.9k • written 2.2 years ago by ▴ 150
1
vote
3
replies
895
views
How to filter .fa file by gene_biotype
rna-seq kallisto
updated 2.3 years ago by 44k • written 2.4 years ago by ▴ 150
0
votes
7
replies
1.4k
views
6 follow
How to create kallisto index that does not include ribosomal genes
enseml kallisto RNA-seq
updated 21 months ago by 44k • written 2.4 years ago by ▴ 150
0
votes
0
replies
411
views
Replicates in sc-RNA seq have different amount of cells-Can I still use them for the analysis?
Cell seurat Single
updated 2.4 years ago by 84k • written 2.4 years ago by ▴ 150
2
votes
4
replies
1.2k
views
Can I use the transcripts_to_genes.txt file from kallisto for bulk RNA seq data?
kallisto
updated 2.5 years ago by 44k • written 2.5 years ago by ▴ 150
2
votes
3
replies
1.3k
views
Ensembl version for mapping and annotating genes
rna-seq kallisto
updated 2.3 years ago by 44k • written 2.5 years ago by ▴ 150
6
votes
2
replies
672
views
Ensembl version
ensembl kallisto
updated 21 months ago by 44k • written 2.5 years ago by ▴ 150
2
votes
2
replies
2.6k
views
Cellranger command for RNA velocity
cellranger Cell velocity RNA Single
updated 2.7 years ago by 9.0k • written 2.7 years ago by ▴ 150
4
votes
2
replies
1.0k
views
Estimated gene counts with tximport
RNA-seq ensembl tximport kallisto
updated 2.3 years ago by 44k • written 2.7 years ago by ▴ 150
3
votes
3
replies
1.4k
views
Will Kallisto show a decrease in the TPM value if I have knocked out 2 of the 8 exons from a gene?
RNA-seq
updated 2.3 years ago by 44k • written 2.8 years ago by ▴ 150
2
votes
2
replies
1.3k
views
How to demultiplex a single indexed library on a dual indexed flow cell?
Cell Single
updated 2.9 years ago by 146k • written 2.9 years ago by ▴ 150
76 results • Page 2 of 2
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