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13
votes
8
replies
2.4k
views
Encountering error when loading scanpy?
scanpy
single-cell
scRNA-seq
7 months ago by
bioinfo
▴ 110
0
votes
0
replies
1.3k
views
Comment:
Comment: How to make a UMAP for single cell data and color cells by average expression of
7 months ago by
bioinfo
▴ 110
0
votes
0
replies
836
views
Comment:
Comment: Problem with tximport and plasmodium falciparum
7 months ago by
bioinfo
▴ 110
0
votes
1
reply
836
views
Comment:
Comment: Problem with tximport and plasmodium falciparum
7 months ago by
bioinfo
▴ 110
1
vote
0
replies
343
views
What is the difference between gseapy.prerank and gseapy.gsea?
gseapy
gsea
7 months ago by
bioinfo
▴ 110
3
votes
2
replies
398
views
Do I need to correct for sequencing depth in bulk RNA seq?
kallisto
RNA-seq
updated 7 months ago by
Ram
40k • written 7 months ago by
bioinfo
▴ 110
0
votes
0
replies
335
views
How to adjust graph in scanpy?
scanpy
single-cell
RNA-seq
7 months ago by
bioinfo
▴ 110
0
votes
0
replies
465
views
How to analyze scRNA seq data with time points (Seurat/Pseudotime)?
monocle3
scRNA-seq
pseudotime
Seurat
R
8 months ago by
bioinfo
▴ 110
0
votes
0
replies
730
views
Comment:
Comment: How to perform scRNA seq analysis?
8 months ago by
bioinfo
▴ 110
2
votes
2
replies
730
views
How to perform scRNA seq analysis?
single-cell
pseudobulk
seurat
8 months ago by
bioinfo
▴ 110
0
votes
0
replies
1.1k
views
Comment:
Comment: Issues with chromosome file in kallisto
9 months ago by
bioinfo
▴ 110
0
votes
1
reply
1.1k
views
Comment:
Comment: Issues with chromosome file in kallisto
9 months ago by
bioinfo
▴ 110
0
votes
0
replies
626
views
Comment:
Comment: Does the bootstrap in kallisto affect the BAM files?
9 months ago by
bioinfo
▴ 110
3
votes
2
replies
626
views
Does the bootstrap in kallisto affect the BAM files?
RNAseq
kallisto
BAM
9 months ago by
bioinfo
▴ 110
1
vote
4
replies
1.1k
views
Issues with chromosome file in kallisto
kallisto
updated 9 months ago by
dsull
★ 4.6k • written 9 months ago by
bioinfo
▴ 110
1
vote
2
replies
522
views
How to run UMAP on single cell results from 2 different runs?
single-cell
seurat
cellranger
updated 9 months ago by
LChart
3.3k • written 9 months ago by
bioinfo
▴ 110
0
votes
1
reply
520
views
Comment:
Comment: How to check gene properties (eg GC, repetitiveness, average expression level)?
10 months ago by
bioinfo
▴ 110
0
votes
1
reply
850
views
Comment:
Comment: Can I demultiplex run based on i5 sequence?
10 months ago by
bioinfo
▴ 110
0
votes
1
reply
850
views
Comment:
Comment: Can I demultiplex run based on i5 sequence?
10 months ago by
bioinfo
▴ 110
1
vote
3
replies
520
views
How to check gene properties (eg GC, repetitiveness, average expression level)?
deseq2
kallisto
RNA-seq
updated 7 months ago by
Ram
40k • written 10 months ago by
bioinfo
▴ 110
0
votes
1
reply
850
views
Comment:
Comment: Can I demultiplex run based on i5 sequence?
10 months ago by
bioinfo
▴ 110
0
votes
1
reply
850
views
Comment:
Comment: Can I demultiplex run based on i5 sequence?
10 months ago by
bioinfo
▴ 110
0
votes
1
reply
850
views
Comment:
Comment: Can I demultiplex run based on i5 sequence?
10 months ago by
bioinfo
▴ 110
0
votes
10
replies
850
views
Can I demultiplex run based on i5 sequence?
demultiplexing
bcl2fastq
updated 10 months ago by
GenoMax
134k • written 10 months ago by
bioinfo
▴ 110
0
votes
1
reply
2.2k
views
Comment:
Comment: How to choose parameters for kallisto single end mode?
10 months ago by
bioinfo
▴ 110
3
votes
13
replies
2.2k
views
How to choose parameters for kallisto single end mode?
kallisto
RNA-seq
updated 4 months ago by
Istvan Albert
98k • written 10 months ago by
bioinfo
▴ 110
0
votes
0
replies
2.2k
views
Comment:
Comment: How to choose parameters for kallisto single end mode?
10 months ago by
bioinfo
▴ 110
7
votes
3
replies
1.3k
views
Should I merge my technical replicates for bulk RNA seq?
RNA-seq
DESeq2
updated 7 months ago by
Ram
40k • written 11 months ago by
bioinfo
▴ 110
4
votes
4
replies
671
views
Can I perform DE analysis with Seurat if I only have one sample per condition?
single
RNA
Seq
cell
seurat
updated 11 months ago by
LChart
3.3k • written 11 months ago by
bioinfo
▴ 110
0
votes
0
replies
251
views
How do I get estimated gene counts for a reference that contains GFP using Kallisto?
gene
ensembl
tximport
kallisto
12 months ago by
bioinfo
▴ 110
0
votes
0
replies
992
views
Comment:
Comment: How can I confirm that I installed bcl2fastq correctly?
12 months ago by
bioinfo
▴ 110
0
votes
0
replies
992
views
Comment:
Comment: How can I confirm that I installed bcl2fastq correctly?
12 months ago by
bioinfo
▴ 110
4
votes
4
replies
992
views
How can I confirm that I installed bcl2fastq correctly?
bcl2fastq
linux
ubuntu
12 months ago by
bioinfo
▴ 110
3
votes
2
replies
501
views
Why do I get a positive gene expression with qPCR for one my genes but in with when doing bulk RNA seq I get a count of 0?
gene
qpcr
kallisto
seq
expression
rna
updated 13 months ago by
dsull
★ 4.6k • written 13 months ago by
bioinfo
▴ 110
1
vote
3
replies
596
views
How to filter .fa file by gene_biotype
rna-seq
kallisto
updated 15 months ago by
Ram
40k • written 16 months ago by
bioinfo
▴ 110
0
votes
1
reply
874
views
Comment:
Comment: How to create kallisto index that does not include ribosomal genes
16 months ago by
bioinfo
▴ 110
0
votes
1
reply
874
views
Comment:
Comment: How to create kallisto index that does not include ribosomal genes
16 months ago by
bioinfo
▴ 110
0
votes
7
replies
874
views
6 follow
How to create kallisto index that does not include ribosomal genes
enseml
kallisto
RNA-seq
updated 9 months ago by
Ram
40k • written 16 months ago by
bioinfo
▴ 110
0
votes
0
replies
298
views
Replicates in sc-RNA seq have different amount of cells-Can I still use them for the analysis?
Cell
seurat
Single
updated 16 months ago by
ATpoint
76k • written 16 months ago by
bioinfo
▴ 110
0
votes
2
replies
834
views
Comment:
Comment: Can I use the transcripts_to_genes.txt file from kallisto for bulk RNA seq data?
17 months ago by
bioinfo
▴ 110
2
votes
4
replies
834
views
Can I use the transcripts_to_genes.txt file from kallisto for bulk RNA seq data?
kallisto
updated 17 months ago by
Ram
40k • written 17 months ago by
bioinfo
▴ 110
0
votes
1
reply
808
views
Comment:
Comment: Ensembl version for mapping and annotating genes
17 months ago by
bioinfo
▴ 110
2
votes
3
replies
808
views
Ensembl version for mapping and annotating genes
rna-seq
kallisto
updated 15 months ago by
Ram
40k • written 17 months ago by
bioinfo
▴ 110
6
votes
2
replies
463
views
Ensembl version
ensembl
kallisto
updated 9 months ago by
Ram
40k • written 17 months ago by
bioinfo
▴ 110
2
votes
2
replies
1.9k
views
Cellranger command for RNA velocity
cellranger
Cell
velocity
RNA
Single
updated 20 months ago by
Friederike
8.8k • written 20 months ago by
bioinfo
▴ 110
4
votes
2
replies
765
views
Estimated gene counts with tximport
RNA-seq
ensembl
tximport
kallisto
updated 15 months ago by
Ram
40k • written 20 months ago by
bioinfo
▴ 110
3
votes
3
replies
1.2k
views
Will Kallisto show a decrease in the TPM value if I have knocked out 2 of the 8 exons from a gene?
RNA-seq
updated 15 months ago by
Ram
40k • written 22 months ago by
bioinfo
▴ 110
0
votes
0
replies
995
views
Comment:
Comment: How to demultiplex a single indexed library on a dual indexed flow cell?
22 months ago by
bioinfo
▴ 110
2
votes
2
replies
995
views
How to demultiplex a single indexed library on a dual indexed flow cell?
Cell
Single
updated 22 months ago by
GenoMax
134k • written 22 months ago by
bioinfo
▴ 110
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