User: DanielC

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DanielC130
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130
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Location:
Canada
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3 weeks, 6 days ago
Joined:
6 years, 4 months ago
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Posts by DanielC

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Comment: C: How to find mutations uniqueness in different resources/databases?
... Thanks! I am checking for the presense of identified novel mutations in all these database to see if they exist in any of them. In addition to this, could you please let me know how can I find the population information these ESR1 mutations information in these databases have come from? Thanks for y ...
written 11 months ago by DanielC130
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How to find mutations uniqueness in different resources/databases?
... Dear Friends, I have identified some mutations in ESR1 gene through Sanger Sequencing. From initial analysis by looking at Ensembl, I see that those mutations are novel (Source mentioned in Ensembl is "dbSNP"). However, I want to check the novelty of these mutations in other mutations/variations da ...
mutation databases clinical written 11 months ago by DanielC130 • updated 11 months ago by Denise CS5.1k
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Comment: C: Is reproducibility of identified variants in a gene important in Sanger sequenci
... Thanks for the answer genomax! ...
written 12 months ago by DanielC130
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Comment: C: Is reproducibility of identified variants in a gene important in Sanger sequenci
... Thanks.. After re-sequencing..if some mutations are not reproduced..do you think they can be studied in silico for protein drug interaction studies? Thanks ...
written 12 months ago by DanielC130
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Comment: C: Is reproducibility of identified variants in a gene important in Sanger sequenci
... Thanks.. Yes, sequenced a sample one time. Yes..referring to reseqencing same sample. Please let me know of your suggestions. Thanks ...
written 12 months ago by DanielC130
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Comment: C: Is reproducibility of identified variants in a gene important in Sanger sequenci
... Thanks, but it does not answer the specific question asked. ...
written 12 months ago by DanielC130
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Is reproducibility of identified variants in a gene important in Sanger sequencing?
... Dear Friends, I have identified some known and novel variants in a gene by sanger sequencing. The identified variants are then used for protein-drug binding interaction studies (this is the main study of the research). Do you think, I should perform the reproducibility check on these identified var ...
sanger sequencing reproducibility variants written 12 months ago by DanielC130
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Comment: C: Crystal structure of lambda phage?
... No, i will see if lambda phage sequence can be found, any idea on this? once we have the sequence we do blast against pdb database; is this what you mean?Thanks ...
written 12 months ago by DanielC130
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Comment: C: Crystal structure of lambda phage?
... Thanks! I am looking for the tail fiber structure of the lambda phage. Could you please let me know about your suggestions on this? ...
written 12 months ago by DanielC130
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Comment: C: how many genomes from BLAST hit should be considered for phylogeny tree analysis
... Thanks! I have performed gene level (using terminase subunit of the genomes) and generated tree from that. Do you think that analysis is considerably OK to predict the family or order the assembled genome belong to? I am working on phages. or use mash distances as a surrogate for sequence iden ...
written 12 months ago by DanielC130

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