User: es

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es30
Reputation:
30
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New User
Location:
United States
Last seen:
6 years, 3 months ago
Joined:
6 years, 7 months ago
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e*************@gmail.com

Posts by es

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(Closed) restriction digest to test for CRISPR disruption
... I have a question about restriction enzymes and their use in helping to ID CRISPR induced mutations.  Once a CRISPR causes an indel at a target sequence, a restriction enzyme can be used to screen several individuals exposed to the CRISPR, screening them for signs of an indel.  I am not clear on a p ...
crispr restriction enzyme sequence genotyping written 6.3 years ago by es30
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Comment: C: How do I calculate the p-value in a gene enrichment analysis, using parametric a
... I used an online exact fisher's test calculator at http://vassarstats.net/index.html, and it said I was using numbers that were too big.  This seemed to agree with what I had read elsewhere, to boot.   I will try a different tool, perhaps R. ...
written 6.6 years ago by es30
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Comment: C: How do I calculate the p-value in a gene enrichment analysis, using parametric a
... And I read that Fisher's ET and the hypergeometric test are equivalent, so long as the FET is one-tailed.  On the other hand, I also read that the hypergeometric test is based on sampling without replacement, whereas FET is not.  I believe I might be reading about these tests in contexts that do no ...
written 6.6 years ago by es30
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Comment: C: How do I calculate the p-value in a gene enrichment analysis, using parametric a
... Thanks.  My data are not expression  data.  Online GE tools are specially designed for gene expression data, and the curated categories do not fit my needs.  This is why i did my own test.  I posted this question to ask how do I go about finding a p-value.  I think finding the expected frequencies f ...
written 6.6 years ago by es30
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How do I calculate the p-value in a gene enrichment analysis, using parametric and non-parametric methods?
... I have a list of 20 QTL from an experiment involving a certain phenotype related to bone development.   Of all the candidate genes within the 20 QTL intervals, 5 are associated with a certain disease in humans.  I tested the set of 20 QTL to see if they are enriched for the disease-associating genes ...
bioinformatics simulation genomics gene enrichment written 6.6 years ago by es30 • updated 6.6 years ago by Devon Ryan98k

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