User: Nowlan Freese
Nowlan Freese • 850
- Reputation:
- 850
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- Location:
- United States
- Last seen:
- 6 days, 8 hours ago
- Joined:
- 5 years, 2 months ago
- Email:
- n************@gmail.com
Posts by Nowlan Freese
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Comment:
C: IGV or IGB
... Both projects are currently [funded][1] and actively [developed][2].
[1]: https://projectreporter.nih.gov/project_info_description.cfm?aid=9505926&icde=43356259&ddparam=&ddvalue=&ddsub=&cr=1&csb=default&cs=ASC&pball=
[2]: https://bitbucket.org/lorainelab/integra ...
written 8 months ago by
Nowlan Freese • 850
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... Hi jpere716,
You should be able to view the [depth graph across the entire chromosome][1].
Can you tell me more about the issue you are having? Is the depth graph not loading across the entire chromosome, or is the top of the depth graph being cut off (the height of of the depth graph can be adjus ...
written 8 months ago by
Nowlan Freese • 850
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... Hi Iraia,
Were you able to figure out the issue? If not, can you send me an image or an example of the data?
Nowlan ...
written 3.3 years ago by
Nowlan Freese • 850
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... Hi Dr. Franco,
The methods that have been recommended to me for analyzing methylation data can be found in two papers: "Human body epigenome maps reveal noncanonical DNA methylation variation" and "Charting a dynamic DNA methylation landscape of the human genome". The supplementary information goes ...
written 3.9 years ago by
Nowlan Freese • 850
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... Interested in finding out how to visualize protein annotations in the context of genomic sequence?
Look for the Integrated Genome Browser at the international Plant and Animal Genome 2016 meeting in San Diego. The IGB team will be giving a live computer demo of the many features in IGB, including ...
written 3.9 years ago by
Nowlan Freese • 850
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... Hi drmoose94,
Yes, it is possible to load the raw data files from AncestryDNA into IGB. You will need to convert the raw data into BED detail format first (https://genome.ucsc.edu/FAQ/FAQformat.html#format1.7), and then you can drag and drop the file directly into IGB.
So here's what my original d ...
written 4.3 years ago by
Nowlan Freese • 850
0
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1
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... Hi Pengcheng Yang,
There is currently no way to design primers from within IGB. My advice would be to use IGB to identify regions of alternative splicing, and then use primer 3 to pick primers.
1) Identify alternative splicing event of interest in IGB.
2) Copy the DNA sequence directly from IGB a ...
written 4.5 years ago by
Nowlan Freese • 850
2
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Answer:
A: Can IGB load BED 6+3 files?
... Hi pgarcia,
The .broadPeak format is not a currently supported file format in IGB, however it can easily be converted to the more common .bed format, which is supported.
To convert the file to a bed file, limit the data to the first 6 columns. To do this on the command line use:
$cut –f1-6 FILE ...
written 4.6 years ago by
Nowlan Freese • 850
3
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1
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... Hi Sarah,
The .wig files are considered graphs in IGB. The equivalent to optimizing stack height for a graph file would be to adjust the Y-axis of the graph so that it is displaying all of the data.
1) Highlight the .wig file track
2) Click on the Graph tab
3) Drag the rightmost slider under the ...
written 4.6 years ago by
Nowlan Freese • 850
2
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2
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1.5k
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... Hi smpyonteck,
The C. elegans genome version in IGB "C_elegans_Oct_2010" is the most recent version, i.e. WS220/ce10. The title within the browser "WS140/ce10" is a typo in our synonym file.
Thank you for finding this!
Nowlan
...
written 4.6 years ago by
Nowlan Freese • 850
Latest awards to Nowlan Freese
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15 months ago,
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For A: IGB- genome sequence in sequence viewer just shows dashes
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17 months ago,
created a question with more than 5,000 views.
For Video: ChIP-Seq Analysis and Visualization using Galaxy and IGB.
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17 months ago,
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For Visualizing protein annotations through ProtAnnot
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2.7 years ago,
created a question with more than 5,000 views.
For Video: RNA-Seq Alignment and Visualization using Galaxy and IGB
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4.3 years ago,
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For Video: Visualization of whole genome DNA methylation data in IGB
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For Video: Intro to All New Features in IGB 8.2
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For A: Where are the different RAM versions of IGB in v8.3.0
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4.6 years ago,
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For (Finished) Live on-line tutorial using Integrated Genome Browser (October 7, 2014 11:30am-12pm EST)
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For A: Where are the different RAM versions of IGB in v8.3.0
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For A: Where are the different RAM versions of IGB in v8.3.0
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For A: IGB- genome sequence in sequence viewer just shows dashes
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For (Finished) Live on-line tutorial using Integrated Genome Browser (October 7, 2014 11:30am-12pm EST)
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For (Finished) Live on-line tutorial using Integrated Genome Browser (November 12, 2014 11:30am-12pm EST)
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For (Finished) Live on-line tutorial using Integrated Genome Browser (November 12, 2014 11:30am-12pm EST)
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For (Finished) Live on-line tutorial using Integrated Genome Browser (October 7, 2014 11:30am-12pm EST)
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For Finished - Live on-line tutorial using Integrated Genome Browser (November 12, 2014 11:30am-12pm EST)
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5.0 years ago,
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For A: IGB- genome sequence in sequence viewer just shows dashes
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For (Finished) Live on-line tutorial using Integrated Genome Browser (October 7, 2014 11:30am-12pm EST)
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For (Finished) Live on-line tutorial using Integrated Genome Browser (October 7, 2014 11:30am-12pm EST)
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