User: Nowlan Freese
Nowlan Freese • 830
- Reputation:
- 830
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- United States
- Last seen:
- 5 months ago
- Joined:
- 4 years, 4 months ago
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Posts by Nowlan Freese
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... Hi Iraia,
Were you able to figure out the issue? If not, can you send me an image or an example of the data?
Nowlan ...
written 2.5 years ago by
Nowlan Freese • 830
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... Hi Dr. Franco,
The methods that have been recommended to me for analyzing methylation data can be found in two papers: "Human body epigenome maps reveal noncanonical DNA methylation variation" and "Charting a dynamic DNA methylation landscape of the human genome". The supplementary information goes ...
written 3.1 years ago by
Nowlan Freese • 830
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... Interested in finding out how to visualize protein annotations in the context of genomic sequence?
Look for the Integrated Genome Browser at the international Plant and Animal Genome 2016 meeting in San Diego. The IGB team will be giving a live computer demo of the many features in IGB, including ...
written 3.1 years ago by
Nowlan Freese • 830
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... Hi drmoose94,
Yes, it is possible to load the raw data files from AncestryDNA into IGB. You will need to convert the raw data into BED detail format first (https://genome.ucsc.edu/FAQ/FAQformat.html#format1.7), and then you can drag and drop the file directly into IGB.
So here's what my original d ...
written 3.4 years ago by
Nowlan Freese • 830
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... Hi Pengcheng Yang,
There is currently no way to design primers from within IGB. My advice would be to use IGB to identify regions of alternative splicing, and then use primer 3 to pick primers.
1) Identify alternative splicing event of interest in IGB.
2) Copy the DNA sequence directly from IGB a ...
written 3.7 years ago by
Nowlan Freese • 830
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Answer:
A: Can IGB load BED 6+3 files?
... Hi pgarcia,
The .broadPeak format is not a currently supported file format in IGB, however it can easily be converted to the more common .bed format, which is supported.
To convert the file to a bed file, limit the data to the first 6 columns. To do this on the command line use:
$cut –f1-6 FILE ...
written 3.7 years ago by
Nowlan Freese • 830
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... Hi Sarah,
The .wig files are considered graphs in IGB. The equivalent to optimizing stack height for a graph file would be to adjust the Y-axis of the graph so that it is displaying all of the data.
1) Highlight the .wig file track
2) Click on the Graph tab
3) Drag the rightmost slider under the ...
written 3.7 years ago by
Nowlan Freese • 830
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... Hi smpyonteck,
The C. elegans genome version in IGB "C_elegans_Oct_2010" is the most recent version, i.e. WS220/ce10. The title within the browser "WS140/ce10" is a typo in our synonym file.
Thank you for finding this!
Nowlan
...
written 3.8 years ago by
Nowlan Freese • 830
1
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... Hi nkm34,
The first thing that comes to mind would be to use the Advanced Search function in IGB to search for your specific motifs.
1. Click on the Advanced Search tab.
2. Select Residues under the Search dropdown menu.
3. Enter your motif(s) - we support use of regular expressions and wild car ...
written 3.8 years ago by
Nowlan Freese • 830
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... Here's a video tutorial on ChIP-seq using Galaxy for data analysis and IGB to visualize the results: https://www.biostars.org/p/126760/
...
written 3.8 years ago by
Nowlan Freese • 830
Latest awards to Nowlan Freese
Great Question
7 months ago,
created a question with more than 5,000 views.
For Video: ChIP-Seq Analysis and Visualization using Galaxy and IGB.
Popular Question
7 months ago,
created a question with more than 1,000 views.
For Visualizing protein annotations through ProtAnnot
Great Question
22 months ago,
created a question with more than 5,000 views.
For Video: RNA-Seq Alignment and Visualization using Galaxy and IGB
Popular Question
3.5 years ago,
created a question with more than 1,000 views.
For Video: Visualization of whole genome DNA methylation data in IGB
Popular Question
3.6 years ago,
created a question with more than 1,000 views.
For Video: Intro to All New Features in IGB 8.2
Scholar
3.7 years ago,
created an answer that has been accepted.
For A: Where are the different RAM versions of IGB in v8.3.0
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For A: Where are the different RAM versions of IGB in v8.3.0
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For A: Where are the different RAM versions of IGB in v8.3.0
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For A: Where are the different RAM versions of IGB in v8.3.0
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For (Finished) Live on-line tutorial using Integrated Genome Browser (October 7, 2014 11:30am-12pm EST)
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For A: Where are the different RAM versions of IGB in v8.3.0
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For A: IGB- genome sequence in sequence viewer just shows dashes
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For (Finished) Live on-line tutorial using Integrated Genome Browser (October 7, 2014 11:30am-12pm EST)
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For (Finished) Live on-line tutorial using Integrated Genome Browser (November 12, 2014 11:30am-12pm EST)
Popular Question
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created a question with more than 1,000 views.
For (Finished) Live on-line tutorial using Integrated Genome Browser (November 12, 2014 11:30am-12pm EST)
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For (Finished) Live on-line tutorial using Integrated Genome Browser (October 7, 2014 11:30am-12pm EST)
Popular Question
4.1 years ago,
created a question with more than 1,000 views.
For Finished - Live on-line tutorial using Integrated Genome Browser (November 12, 2014 11:30am-12pm EST)
Teacher
4.1 years ago,
created an answer with at least 3 up-votes.
For A: IGB- genome sequence in sequence viewer just shows dashes
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For (Finished) Live on-line tutorial using Integrated Genome Browser (October 7, 2014 11:30am-12pm EST)
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For (Finished) Live on-line tutorial using Integrated Genome Browser (October 7, 2014 11:30am-12pm EST)
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