User: Anima Mundi

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Anima Mundi2.3k
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Posts by Anima Mundi

<prev • 316 results • page 1 of 32 • next >
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Comment: C: Find all sequence of a specific genus amplified by a couple of primers
... Assuming you performed your PCR on cDNA, checking for amplicons from genome is merely a way to check for potential sources of genomic contamination. Doing this using your whole amplicon would just return genomic locations in which your full amplicon (or something thereof) is present, if any. For ins ...
written 8 weeks ago by Anima Mundi2.3k
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Answer: A: Find all sequence of a specific genus amplified by a couple of primers
... Hello, a rapid way to do that is to: 1) reverse-complement (RC) your right primer 2) build your subject database as a FASTA file (if needed) 3) go to [NCBI nucleotide BLAST][1] 4) paste your left primer, type a series of Ns right next to it, then paste your RC right primer 5) load your databas ...
written 8 weeks ago by Anima Mundi2.3k
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Comment: C: transition to bioinformatics
... Hi, just a quick comment. > But I really want this, I really like it. Just roll with it ;) Nowadays there is a strong need for bioinformaticians, if you happen to have a strong interest for bioinformatics and you are fresh from a degree in biology then you are in luck, in my opinion. ...
written 9 weeks ago by Anima Mundi2.3k
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Comment: C: Change nexus textlabels
... Yep, indeed the script was simply meant to help you re-format your list IDs, as you originally requested. If you want to get some help then... you should help people to help you! For instance, in the original question at least you were pointing out to one example ID. That said, unfortunately I am no ...
written 11 weeks ago by Anima Mundi2.3k
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Comment: C: Change nexus textlabels
... Well, this was meant to reply to an original question, now missing -.-'' ...
written 11 weeks ago by Anima Mundi2.3k
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Answer: A: Change nexus textlabels
... Hi, if you want to try replacing headers, this should do the job (Python 2.7 script, takes the two list filenames as arguments): import sys listA = [] listB = [] for line in open(sys.argv[1]): listA.append(line.replace('\n','')) for line in open(sys.argv[2 ...
written 11 weeks ago by Anima Mundi2.3k
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Answer: A: Finding restriction sites when the gene is on the negative strand
... Hi, welcome. To make your restriction map: a) consider the coordinates of your gene irrespective of its sense strand, i.e. `chr11:355953-356144` b) it is useful to expand its coordinates a bit both upstream and downstream (because you are interested in restriction sites surrounding it); say, we e ...
written 12 weeks ago by Anima Mundi2.3k
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Comment: C: DNA Motif discovery from a large number of FASTA sequences
... I see, then I think you should be just fine with the MEME suite, what is your problem exactly? ...
written 4 months ago by Anima Mundi2.3k
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Comment: C: DNA Motif discovery from a large number of FASTA sequences
... Hello, did you attempt to use MEME via command line or the web version of it? In the second case, I would just recommend switching to command line. ...
written 4 months ago by Anima Mundi2.3k
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Comment: C: What does a recruiter expect from a Bioinformatician ?
... Just for the fun of discussing: what I see is that a recurrent theme among "hard" scientists (i.e. those having a background in physical - as opposed to natural - sciences) is that biology can be approximated to a list of notions. I disagree with that view: while notions in biology are certainly imp ...
written 5 months ago by Anima Mundi2.3k

Latest awards to Anima Mundi

Epic Question 15 days ago, created a question with more than 10,000 views. For How To Use Bedtools To Extract Promoters From A Mouse Bed File
Epic Question 19 days ago, created a question with more than 10,000 views. For How To Use Bedtools To Extract Promoters From A Mouse Bed File
Popular Question 6 weeks ago, created a question with more than 1,000 views. For How To Download All The Introns From Ensembl
Scholar 12 weeks ago, created an answer that has been accepted. For A: Retrieving RNA sequence from corresponding genomic coordinates
Great Question 4 months ago, created a question with more than 5,000 views. For How To Use Bedtools To Extract Promoters From A Mouse Bed File
Popular Question 5 months ago, created a question with more than 1,000 views. For Meme-Suite Is Down... Does Anybody Know What'S Happening?
Popular Question 5 months ago, created a question with more than 1,000 views. For How To Use Bedtools To Extract Promoters From A Mouse Bed File
Popular Question 5 months ago, created a question with more than 1,000 views. For How To Use Bedtools To Extract Promoters From A Mouse Bed File
Popular Question 5 months ago, created a question with more than 1,000 views. For How To Use Bedtools To Extract Promoters From A Mouse Bed File
Great Question 6 months ago, created a question with more than 5,000 views. For How To Use Bedtools To Extract Promoters From A Mouse Bed File
Commentator 6 months ago, created a comment with at least 3 up-votes. For C: Should Fasta Be Capitalized?
Popular Question 8 months ago, created a question with more than 1,000 views. For How To Use Bedtools To Extract Promoters From A Mouse Bed File
Popular Question 8 months ago, created a question with more than 1,000 views. For How To Use Bedtools To Extract Promoters From A Mouse Bed File
Teacher 8 months ago, created an answer with at least 3 up-votes. For A: Is A Gap At The Same Position In Query And Template Possible?
Good Answer 9 months ago, created an answer that was upvoted at least 5 times. For A: Is A Gap At The Same Position In Query And Template Possible?
Popular Question 9 months ago, created a question with more than 1,000 views. For Meme-Suite Is Down... Does Anybody Know What'S Happening?
Popular Question 9 months ago, created a question with more than 1,000 views. For How To Use Bedtools To Extract Promoters From A Mouse Bed File
Popular Question 9 months ago, created a question with more than 1,000 views. For How To Display Old Assemblies Of The Mouse Genome In The Ensembl Genome Browser
Librarian 12 months ago, created a post with more than 10 bookmarks. For Useful Bash Commands To Handle Fasta Files
Popular Question 13 months ago, created a question with more than 1,000 views. For Gene Coordinates For A Gene Family Members From The Mouse Genome
Great Question 14 months ago, created a question with more than 5,000 views. For How To Use Bedtools To Extract Promoters From A Mouse Bed File
Popular Question 14 months ago, created a question with more than 1,000 views. For Literature-Based Interactomics Tool
Popular Question 14 months ago, created a question with more than 1,000 views. For Gene Coordinates For A Gene Family Members From The Mouse Genome
Commentator 15 months ago, created a comment with at least 3 up-votes. For C: Should Fasta Be Capitalized?
Popular Question 15 months ago, created a question with more than 1,000 views. For Gene Coordinates For A Gene Family Members From The Mouse Genome

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