User: guoql.karen

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guoql.karen10
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4 years, 10 months ago
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Posts by guoql.karen

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Comment: C: Changing Fasta Header
... This perl script may help to solve your porblem: open F,"new_genome.fa"; while(){ print OUT $_ unless ($_=~/^>/); print OUT "$1\n" if ($_=~/^(>.*)\s+range.*/); } close(F);close(OUT); ...
written 3.3 years ago by guoql.karen10
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Comment: C: htseq-count result for strand specific RNA-seq data using three "-s" mode
... OK! Thank you for your comments! It's helpful! ...
written 3.3 years ago by guoql.karen10
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htseq-count result for strand specific RNA-seq data using three "-s" mode
... Hey there! Could anyone give some explainations for the htseq-count result here: 1. **DATA** - SAM file produced by mapping RNA-seq data (fr-secondstrand) to the genome. - GTF filE 2 **STEPS** - use samtools to sort the BAM file by name, and converse it to SAM file - use htseq-count and s ...
next-gen rna-seq written 3.3 years ago by guoql.karen10 • updated 3.3 years ago by Carlo Yague4.7k
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Answer: A: query on micro RNAs(miRNAs)
... Hi, It is supposed to be no more than 2 mismatches of miRNA candidates aligned to the known miRNAs mature sequences.I agreed with Dr.Lorena Pantano, and you should attach the the description about your query in the paper.Then we can give a precise explanation of the meaning. Otherwise, you can emai ...
written 4.8 years ago by guoql.karen10

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