User: benjaminmorgan2016

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New User
Location:
United States
Last seen:
4 years, 11 months ago
Joined:
5 years ago
Email:
b*****************@u.northwestern.edu

Posts by benjaminmorgan2016

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Comment: C: Trimmomatic not removing TruSeq3 adapters
... Correct.  My point was just that the fragments being sequenced should all be long enough that a read from any one direction should not ever be long enough to read through into the RC adapter sequence from the opposite end of the fragment.  I am trying to get Trimmomatic to recognize the the adapters ...
written 5.0 years ago by benjaminmorgan201630
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Comment: C: Trimmomatic not removing TruSeq3 adapters
... Hi Brian, Thanks for the reply.  I'm going to go ahead and try with the rc, but I don't think it's likely to solve the problem (you'll hear from me real fast if it does), since I am not anticipating any read-through: the forward and reverse reads are separated by a large enough insert (size unknown ...
written 5.0 years ago by benjaminmorgan201630
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Comment: C: Trimmomatic not removing TruSeq3 adapters
... Hi Istvan, Thanks for the quick reply (I'm obviously waiting with bated breath for responses).   Maybe I'm totally misunderstanding how Trimmomatic works, but that does not make sense to me.  Why should an entire read be dropped because there is an adapter match?   My understanding based on the m ...
written 5.0 years ago by benjaminmorgan201630
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Trimmomatic not removing TruSeq3 adapters
... Hi, I'm working on putting together exercises in sequence data processing and analysis for graduate and undergraduate students, most of whom have relatively little experience in this area.  One of the first things I'd like to have them do is remove adapter sequences and quality trim, and I've been ...
adapter trimming trimmomatic next-gen sequencing written 5.0 years ago by benjaminmorgan201630 • updated 4.5 years ago by simon.rayner20

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