User: silvia.caprari84

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Germany
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Posts by silvia.caprari84

<prev • 28 results • page 1 of 3 • next >
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Answer: A: why the repeat sequences interfere with the assembly of the contigs
... Thank you both of you for yourclear answers.One thing is still unclear. With the pair-end sequencing do you have the sequencing of the central part?I mean, your reads sequence the ends if I understood correctly..but what about the central parts? ...
written 4.3 years ago by silvia.caprari8450
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why the repeat sequences interfere with the assembly of the contigs
... Hi , can you explain in a very very simple way why the repeat sequences interfere with the assembly of the contigs in the sequencing? I can't figure it out. And why the pair-end sequencing helps to overcome this problem?I read that this involves the sequencing of both ends of a fragment, ..but I ca ...
sequence assembly next-gen sequencing written 4.3 years ago by silvia.caprari8450
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Answer: A: finishing a genome from assembly of contigs
... Thank you very much dago ...
written 4.3 years ago by silvia.caprari8450
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Answer: A: finishing a genome from assembly of contigs
... yes, really sorry about that. then can I just ask just your opinion about this: if I run Blast by using a sequence of a known protein as a query against a file containing all the contigs, the known sequence matches more contigs, and most often the same sequence in different contigs can be different ...
written 4.3 years ago by silvia.caprari8450
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finishing a genome from assembly of contigs
... I all, I am new in analysing sequencing results coming from ngs and I sent some clinical bacterial isolates (they are likely to have plasmids)to be sequenced with Illumina. I anticipate that I am completely new with the terminology , methodology and everything else..I got files named "reads" and fil ...
genome assembly next-gen blast sequencing written 4.3 years ago by silvia.caprari8450 • updated 4.3 years ago by sidrairshad290
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Answer: A: How to determine the sequence of the plasmids from ngs results?
... so, I don't know if I am talkin about fantasy..but could it have been the result of more copies of the same plasmid in the cell? some of these copies underwent to mutations and some other didn't? ...
written 4.3 years ago by silvia.caprari8450
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Answer: A: How to determine the sequence of the plasmids from ngs results?
... Ok. I will try.Can I ask why I find contigs that match the same known sequence (when I run Blast) but they show slightly differences in terms of their nucleotide sequence? For example I have two contigs that match the known sequence for the same region, one matches it with 100% identity , the other ...
written 4.3 years ago by silvia.caprari8450
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How to determine the sequence of the plasmids from ngs results?
... Hi all, I am trying to interpret the results coming from ngs on some bacterial isolates that are antibiotic resistant. This is completely new for me and I have different questions about it.I would like to know; how I can reconstruct the sequence of the plasmids starting from the contigs I have? I m ...
gene next-gen alignment blast sequencing written 4.3 years ago by silvia.caprari8450
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analysis of ngs results
... Hi, i anticipate I am completely new with sequencing and bioinformatics in general. I have results from next generation sequencing on bacteria genomes I sent off for sequencing. The bactyeria are antibioitc resistant and they should carry their resistance genes on plasmids. The first thing that is ...
next-gen written 4.3 years ago by silvia.caprari8450
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How to distinguish plasmids and chromosome when I analyze data from next generation sequencing?
... Hi all, I need to analyse results coming from next generation sequencing on bacterial 3 strains that are carbapenem-resistant. I am using the software "Artemis" to analyze the sequences. I anticipate that this stuff is totally new for me. I am particularly interested in the resistance genes and in ...
gene next-gen sequencing written 4.3 years ago by silvia.caprari8450 • updated 4.3 years ago by YaGalbi1.5k

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