User: Roger

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Roger0
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Posts by Roger

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Bowtie2 local alignment SAM file SEQ output - reverse complement or not ?
... Hi all,  According to the Bowtie2 manual, Bowtie2 writes the reverse complement sequence of the original read into the SAM file output if the read maps to the reverse complement of the reference (--> negative strand, FLAG = 16).  Cited from the manual: 10. Read sequence (reverse-complemented i ...
sam strandspecificity strandness seq bowtie2 written 4.5 years ago by Roger0 • updated 4.5 years ago by Istvan Albert ♦♦ 81k

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