Moderator: Josh Herr

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Josh Herr5.5k
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University of Nebraska
Website:
http://joshuaherr.com/
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@number_three
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I'm an assistant professor in the Department of Plant Pathology and The Center for Plant Science Innovation at the University of Nebraska.

You can read more about me and my interests at my blog Cyme & Cystidium or at my personal website or the website of my research lab.

You can also find me at Github, Software & Data Carpentry, and Twitter.

Posts by Josh Herr

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Comment: C: comparison of species profiling simply using 16s rRNA in 16s rRNA amplicon seqeu
... Thank you!  I'm a little clearer on your question now.  I was unaware of these tools. ...
written 2.2 years ago by Josh Herr5.5k
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Comment: C: comparison of species profiling simply using 16s rRNA in 16s rRNA amplicon seqeu
... Thanks for the information -- would you mind posting the references (links?) about the mOTU tool and the use of the chaperone Cpn60 for species profiling?  I'm not familiar with either of these.  This may help others who have the same question you do with regards to this. Thanks again for your ques ...
written 2.2 years ago by Josh Herr5.5k
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Comment: C: comparison of species profiling simply using 16s rRNA in 16s rRNA amplicon seqeu
... No problem, it's my pleasure.  Just letting you know about S as being a Svedberg unit -- using the correct terminology will show that you understand concepts to other people. I'm unsure what you mean when you use "mOTU" here, which typically means "molecular operational taxonomic unit" which is arb ...
written 2.2 years ago by Josh Herr5.5k
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Answer: A: comparison of species profiling simply using 16s rRNA in 16s rRNA amplicon seqeu
... I'm not quite sure I fully understand your question -- maybe this is not obvious to you, but using markers for selective sequencing and random whole genome shotgun sequencing will not inherently capture the same sequences (or sequence diversity).  One is specific (16S) and the other is random (metag ...
written 2.2 years ago by Josh Herr5.5k
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Comment: C: Help in sff file format processing
... Great - Glad it worked out! ...
written 2.3 years ago by Josh Herr5.5k
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Comment: C: Help in sff file format processing
... Hello lzheng.chn I'm sorry to hear that you are having this problem -- I'm not sure what has caused it.  It might help if you add that sequence (AGAGCGAA) from all your samples to the oligo file that you provide in mothur to have that region trimmed during the shhh.flow step.   You can alternately ...
written 2.3 years ago by Josh Herr5.5k
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Answer: A: Help in sff file format processing
... I'm a little confused what is actually happening here -- not sure what you are actually seeing and if it is an artifact of your sequencing or a sequence processing error. Since it seems like you are running your sequences through mothur, I would use the Trim.seqs command (see wiki here). If you're ...
written 2.3 years ago by Josh Herr5.5k
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Answer: A: Suggested Undergraduate Courses to become a Bioinformatician
... This is difficult to answer because we don't know which courses are offered to you in your third and fourth years.  I would try to find a good advisor or mentor to guide you to your local university environment in Hamilton (this might not be the advisor who is assigned to you; this was the case for ...
written 2.4 years ago by Josh Herr5.5k
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Comment: C: How to calculate overall coverage in de novo metagenomics assembly?
... One more time: Is this a metagenome (unknown reference) or a synthetic microbial community (known reference genomes)?  This matters here if you can use k-mers or not to estimate coverage. After your last comment here, I'm just confused what exactly you are looking to do.  What is your research ques ...
written 2.4 years ago by Josh Herr5.5k
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Comment: C: How to calculate overall coverage in de novo assembly?
... Still not sure if you're talking genome or metagenome assembly -- this matters on the issue as they are not the same. If you look at the link I posted from a previous questions about Ray, you'll see it uses k-mers to measure coverage.  Don't confuse k-mer coverage with actual read coverage as strai ...
written 2.4 years ago by Josh Herr5.5k

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