User: Joshi

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Joshi0
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Posts by Joshi

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Query regarding chr6_dbb_hap3
... Hi, I've been doing some analysis of exons/introns based on the hg19 assembly and RefSeq annotation track from UCSC. A number of chromosomes represent [haplotype alleles or unplaced contigs][1]. If doing analysis of exons/introns ... (e.g. avg size of introns across chr6 / chr7 as an example) - s ...
annotation refseq written 4 days ago by Joshi0
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Calculating coverage for WGS, WES and RNASeq
... Hi, I need some advise on calculating coverage Basically for a set of BAM files, WGS, WES and RNA-Seq - I am interested in calculating and representing coverage - to show the contrast between these differing sequencing techniques (and limited to that ... not comparison). The coverage needs to be c ...
wgs sequence coverage wes rna-seq written 9 weeks ago by Joshi0 • updated 9 weeks ago by Philipp Bayer4.8k
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Appropriate application of RPKM
... Hi, Would appreciate any advice on this particular topic... Having seen examples of RPKM calculations and what they signify - I'm not entirely clear whether it's an appropriate metric for exome sequencing coverage. From what I understand, it's appropriate when trying to compare relative transcript ...
rpkm wes rna-seq written 17 months ago by Joshi0 • updated 17 months ago by Damian Kao14k

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