User: brunobsouzaa

gravatar for brunobsouzaa
brunobsouzaa490
Reputation:
490
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Trusted
Location:
Brazil
Last seen:
23 hours ago
Joined:
5 years, 7 months ago
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b***********@gmail.com

Posts by brunobsouzaa

<prev • 115 results • page 2 of 12 • next >
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Comment: C: Gene Set Enrichment Analysis
... Thank you very much for this tutorial. Just one question, when I run fgseaRes <- fgseaMultilevel(pathways=pathways.hallmark, stats=ranks) I'm getting the following message: Error in mcexit(0L) : write error, closing pipe to the master Do you what this could be? Thanks ...
written 7 weeks ago by brunobsouzaa490 • updated 6 weeks ago by _r_am32k
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Comment: C: Is trimming necessary for RNAseq?
... I don't perform trimming at all but I do apply some filters for genes with really low read counts. Also, a results validation helps a lot! But, you can perform some tests and compare results with trimmed and not trimmed data, this will give you a really good direction on what to do! ...
written 9 weeks ago by brunobsouzaa490
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Comment: C: Calling CNVs from SNPs within targeted gene panel
... I work with targeted sequencing and I like to use `cnvkit` and `ExomeDepth` for CNV calls. Take a look into their respective manuals, might help you! `convading` is also a good tool, but it's too slow! ...
written 9 weeks ago by brunobsouzaa490
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Comment: C: does RSEM need STAR?
... See [here][1] [1]: https://github.com/bli25broad/RSEM_tutorial ...
written 9 weeks ago by brunobsouzaa490
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Comment: C: Why is gnomAD AF vs gnomAD exome AF so different?
... Actually not. Take a look at gnomAD FAQ. The number of samples in Exome and Genome studies is very different! ...
written 10 weeks ago by brunobsouzaa490
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Comment: C: Why is gnomAD AF vs gnomAD exome AF so different?
... Perhaps you're looking at genome and exome AF! ...
written 10 weeks ago by brunobsouzaa490
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Answer: C: Convert CSV to PED
... Taken from [here][1] If your .csv file contains data reqired for .ped and .map formats you can use it directly. For the .ped mandatory columns are: Family ID, Individual ID, Paternal ID, Maternal ID, Sex (1=male; 2=female; other=unknown), Phenotype. You need these data to run Plink. Then instead of ...
written 10 weeks ago by brunobsouzaa490
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Comment: C: Annotating variants using latest version fof the genome
... If you use different genome versions yes, it will give you wrong annotations! ...
written 12 weeks ago by brunobsouzaa490
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Answer: C: Annotating variants using latest version fof the genome
... Just to check if I understood, you have SNP data with hg19 coordinates and you want to annotate with hg38 coordinates? Don't do this, use the same genome version to annotate your files. ...
written 12 weeks ago by brunobsouzaa490
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Comment: C: problem with a for loop
... Oh, now I got it! You can simply remove the intermediate files as soon as they are used (I do this!) or you can try to use pipe `|` between your functions. I've never tried this approach but doesn't hurt to try! ...
written 3 months ago by brunobsouzaa490

Latest awards to brunobsouzaa

Scholar 7 weeks ago, created an answer that has been accepted. For A: Bash script for calling CNVs using cnvkit
Teacher 7 weeks ago, created an answer with at least 3 up-votes. For A: How to analyze Illumina RNASeq data
Centurion 10 weeks ago, created 100 posts.
Popular Question 10 weeks ago, created a question with more than 1,000 views. For Trying to visualize .bed file
Supporter 3 months ago, voted at least 25 times.
Scholar 3 months ago, created an answer that has been accepted. For A: Bash script for calling CNVs using cnvkit
Teacher 3 months ago, created an answer with at least 3 up-votes. For A: How to analyze Illumina RNASeq data
Teacher 5 months ago, created an answer with at least 3 up-votes. For A: How to analyze Illumina RNASeq data
Scholar 18 months ago, created an answer that has been accepted. For A: Bash script for calling CNVs using cnvkit
Popular Question 4.5 years ago, created a question with more than 1,000 views. For Trying to visualize .bed file
Popular Question 4.5 years ago, created a question with more than 1,000 views. For Doubt on filtering data from .VCF file

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