User: alyssamolinaro91

Reputation:
10
Status:
New User
Location:
Canada
Last seen:
3 years, 9 months ago
Joined:
4 years, 11 months ago
Email:
a***************@gmail.com

Posts by alyssamolinaro91

<prev • 2 results • page 1 of 1 • next >
0
votes
3
answers
6.0k
views
3
answers
Comment: C: Tools for demultiplexing a large fastq file based on random in-line barcodes
... Hi Igor, your zcat solution appears to have done the trick. I used it to generate a barcode list, which I then used as the barcode input file for fastx_barcode_splitter (http://hannonlab.cshl.edu/fastx_toolkit/commandline.html). I now have demultiplexed fastq files. One strange thing though, when I ...
written 3.8 years ago by alyssamolinaro9110
4
votes
3
answers
6.0k
views
3
answers
Tools for demultiplexing a large fastq file based on random in-line barcodes
... Hi all, I will be using Drop-seq to prepare cDNA libraries for thousands of single cells, followed by single-end sequencing on a HiSeq2500 (read length = 100 bases). This will involve the addition of a unique 12 nucleotide cell barcode to the 5' end of all reads originating from the same cell (so b ...
drop-seq rna-seq demultiplexing written 3.8 years ago by alyssamolinaro9110 • updated 2.6 years ago by Biostar ♦♦ 20

Latest awards to alyssamolinaro91

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1552 users visited in the last hour