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Posts by alyssamolinaro91
... Hi Igor, your zcat solution appears to have done the trick. I used it to generate a barcode list, which I then used as the barcode input file for fastx_barcode_splitter (http://hannonlab.cshl.edu/fastx_toolkit/commandline.html). I now have demultiplexed fastq files. One strange thing though, when I ...
... Hi all, I will be using Drop-seq to prepare cDNA libraries for thousands of single cells, followed by single-end sequencing on a HiSeq2500 (read length = 100 bases). This will involve the addition of a unique 12 nucleotide cell barcode to the 5' end of all reads originating from the same cell (so b ...
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