User: b.schiffthaler

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Posts by b.schiffthaler

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Comment: C: RNA-seq expression patterns
... You may also want to check out [mfuzz](https://bioconductor.org/packages/release/bioc/html/Mfuzz.html) or [WGCNA](https://cran.r-project.org/web/packages/WGCNA/index.html). These are straight-up clustering approaches on your whole dataset. You could always limit the input to significant as per LRT, ...
written 8 weeks ago by b.schiffthaler20
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Answer: A: Problem in understanding False Discovery Rate (FDR)
... No statistical test is 100% accurate, it's a probability. So let's say you test if two means are significantly different from each other. Your test tells you to reject the null hypothesis with P=0.05. But this test isn't absolute, it also has inherent error. Let's say you are wrong once in ten tests ...
written 10 weeks ago by b.schiffthaler20
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Answer: A: assembly without trimming vs with trimming
... You should always trim adapters and other foreign sequence (if you know possible sources of contaminants). Any trimming beyond that (quality trimming) is likely going to degrade the quality of your assembly. You can trim _very_ mildy, but even there I'd be careful. Here's a [paper](https://www.fron ...
written 10 weeks ago by b.schiffthaler20

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