User: micromira

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micromira0
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Posts by micromira

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RNA seq data validation with qPCR: compare log Fold change vs Fold change?
... I sent my RNA seq for analysis to our collaborators, who returned me data in format:  log fold change/FDR/pValue. I took a gene which is upregulated in treated sample, LgFC= 1.2, FDR, pValue < 0.05 and run validation by RT-PCR. Using ddCt method I got 1.3 Fold change difference.   How do I co ...
rna-seq rt-pcr written 5.2 years ago by micromira0 • updated 5.2 years ago by Devon Ryan98k

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