User: Urja

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Urja0
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Posts by Urja

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Answer: A: Paired-end RNASeq insert length for de novo assembly using Oases
... Thank you. I will align reads to the assembled transcriptome and check for overlaps. Any idea how oases deals with paired end reads if no insert size is specified? ...
written 3.1 years ago by Urja0
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Answer: A: Paired-end RNASeq insert length for de novo assembly using Oases
... That's a good idea but unfortunately, no reference genome is available. I noticed that even without giving an insert size Oases runs and does an assembly, but I am not sure if it considers the paired information or just treats them as single end reads. Any thoughts on that ? Thanks a lot for your ...
written 3.1 years ago by Urja0
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Paired-end RNASeq insert length for de novo assembly using Oases
... Hi Fellow Bioinformatician, I am doing de novo RNASeq assembly using Velvet/Oases for a few publicly available RNASeq datasets. They are paired end 101bp reads generated by Illumina. I read and found that Oases has a parameter for insert length (-ins_length), but I have no idea how much the insert ...
de novo assembly rna-seq oases insert length written 3.1 years ago by Urja0

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