User: pablominguez

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pablominguez10
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3 years, 9 months ago
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3 years, 11 months ago
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Posts by pablominguez

<prev • 4 results • page 1 of 1 • next >
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Comment: A: Illumina NextSeq SampleSheets
... Perfect, both solutions seems to work. Thanks a lot! ...
written 3.8 years ago by pablominguez10
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Illumina NextSeq SampleSheets
... Hi all, I have a illumina run with several projects inside (TruSeq Amplicon and Nextera Rapid Capture) and I cannot find how to configure the SampleSheet.csv to include for example the Assay type which are different or the adpaters. I just want to run bcl2fastq and at the moment I am creating separ ...
ngs next-gen written 3.8 years ago by pablominguez10
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Comment: C: HTSeq-count paired-end --stranded=yes gives less counts than --stranded=no
... Thanks a lot for the suggestion, I have run Kallisto and the numbers are similar to --stranded=no. ...
written 3.9 years ago by pablominguez10
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HTSeq-count paired-end --stranded=yes gives less counts than --stranded=no
... Hi all, I'm confused on how should I call htseq-count for my dataset concerning option --stranded [yes or no]. I'm analysing paired-end RNASeq data, at the alignment step with tophat I produced one single bam file from the two reads (R1, R2). So I have one bam file with both strands, this file is ...
rna-seq written 3.9 years ago by pablominguez10 • updated 3.9 years ago by Devon Ryan94k

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