User: modihardikv

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Posts by modihardikv

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Comment: A: Raw Read Quality Filtering using FASTX-Toolkit of miRNA-Seq data
... 1. Yes, it is right to use miRBase as reference. However we have decided to use v20.. 2. Are these alignment is on total reads or on collapsed reads? 3. We are also working on optimizing alignment tool, (Novoaligner,bowtie, bwa). Thanks for your quick response. ...
written 3.1 years ago by modihardikv0
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Comment: A: Raw Read Quality Filtering using FASTX-Toolkit of miRNA-Seq data
... Thanks Jimmy, Please post your answer. ...
written 3.1 years ago by modihardikv0
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Raw Read Quality Filtering using FASTX-Toolkit of miRNA-Seq data
... I am analyzing miRNA-Seq data for differential expression analysis of miRNAs. First step in the process, I am performing is raw read quality filtering using FASTX-Toolkit to filter out reads with poor qualities using the following settings: 1. the minimum quality score for each base = 20; 2. ...
next-gen mirna-seq rna-seq written 3.1 years ago by modihardikv0

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