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- 1 year, 5 months ago
Posts by sHeyne
Answer: A: Strange MA-plot using DESeq2
... The red cloud at the bottom looks like a contamination from some other cell/tissue type (in case of eg. mouse or human). In your case, all samples/replicates are from "whole body" beetles? Don't know how this then could happen... In order to see if this effect relates to one or more replicates, you ...
... Methods like Salmon seem powerful and they overall do a good job, however recently I stumbled upon the (at least Salmon's) behavior on "multi" vs "uniquely" mapping reads (in featureCounts/htseq-count terms, Salmon uses SMEMs/k-mers). Eventhough there are some parameters to influence salmon like ...
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