User: sHeyne

gravatar for sHeyne
sHeyne30
Reputation:
30
Status:
New User
Location:
Freiburg, Germany
Last seen:
3 months ago
Joined:
1 year, 8 months ago
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Posts by sHeyne

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Answer: A: Strange MA-plot using DESeq2
... The red cloud at the bottom looks like a contamination from some other cell/tissue type (in case of eg. mouse or human). In your case, all samples/replicates are from "whole body" beetles? Don't know how this then could happen... In order to see if this effect relates to one or more replicates, you ...
written 3 months ago by sHeyne30
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Comment: C: Current state-of-the-art for RNA-Seq gene / transcript expression quantification
... Methods like Salmon seem powerful and they overall do a good job, however recently I stumbled upon the (at least Salmon's) behavior on "multi" vs "uniquely" mapping reads (in featureCounts/htseq-count terms, Salmon uses SMEMs/k-mers). Eventhough there are some parameters to influence salmon like ...
written 20 months ago by sHeyne30

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