User: nkabo

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nkabo50
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Posts by nkabo

<prev • 44 results • page 1 of 5 • next >
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Comment: C: How to obtain cell or tissue-specific enrichment of a list of genes
... Thank you for your reply, I will try obtain control data coming from leukocytes. ...
written 3 days ago by nkabo50
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Comment: C: How to search for gene isoforms in FASTQ file
... Thanks a lot, it worked. ...
written 3 days ago by nkabo50
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Comment: C: How to obtain cell or tissue-specific enrichment of a list of genes
... Data for case and control samples are from different types of cells (case is from leukocytes and control is from endothelial cells). I have to compare case and control for differential gene expression analysis. Due to low number of controls and cases, I have only these two samples (1 for case and 1 ...
written 3 days ago by nkabo50
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How to search for gene isoforms in FASTQ file
... Dear all, I am a very beginner of RNA-seq analysis. I had completed the RNA-seq process by following the workflow in the link below; I had used Salmon and tximport: https://bioconductor.org/packages/release/bioc/vignettes/tximport/inst/doc/tximport.html After this part, I had used Ensembl for tra ...
fastq isoform identification rna-seq written 3 days ago by nkabo50 • updated 3 days ago by ATpoint41k
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Comment: C: How to obtain cell or tissue-specific enrichment of a list of genes
... Thank you for your reply. I will check Human Cell Atlas. The reason why I would like to find cell-type specific genes is that I have RNA-Seq data from 2 different cell types (one is from leukocytes and other is from endothelial cells) and I would like to compare these 2 datasets. Since there is lack ...
written 3 days ago by nkabo50
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Comment: C: How to obtain cell or tissue-specific enrichment of a list of genes
... Thank you for your response. I have had fastq files from RNA-Seq, the final result file is a txt file involved gene names, samples and expression values. The data is from endothelial cells, so I would like to exclude genes that: - differentially highly expressed in endothelial cells compared to o ...
written 11 days ago by nkabo50
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How to obtain cell or tissue-specific enrichment of a list of genes
... Dear all, I have a list of genes, composed of over 19 000 rows. I would like to give this list as an input and get which tissues or cell types that these genes are enriched at most and at least as an output. Ideally, I would like to see which genes are enriched in which cell-type or tissue-type. Is ...
R gene enrichment written 5 weeks ago by nkabo50
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Comment: C: How to find cell-type specific gene expression signatures
... Thank you for your response, the study had completed before I started working on unfortunately, so I could not have much effect on study design. ...
written 5 weeks ago by nkabo50
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Comment: C: How to find cell-type specific gene expression signatures
... Thank you for your response, I will talk to my PI about the situation. ...
written 5 weeks ago by nkabo50
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Comment: C: How to find cell-type specific gene expression signatures
... Thank you for your response. Unfortunately, we do not have replicates neither and we do not have the possibility of producing RNA-seq data from healthy subjects. I considered getting 1. RNA-seq data from lymphocytes of a healthy child 2. RNA-seq data from endothelial cells of a healthy adult f ...
written 5 weeks ago by nkabo50

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