User: Torst
Torst • 960
- Reputation:
- 960
- Status:
- Trusted
- Location:
- Australia
- Website:
- http://www.bioinformat...
- Last seen:
- 1 year, 3 months ago
- Joined:
- 9 years, 2 months ago
- Email:
- t**************@gmail.com
Microbial bioinformatics, *-omics.
Posts by Torst
<prev
• 100 results •
page 1 of 10 •
next >
0
votes
1
answer
978
views
1
answers
... `Score.value.real` suggests maybe you should add an `-evalue 1E-3` or something?
As an aside, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848038/ is a good article on matrices for short queries.
...
written 17 months ago by
Torst • 960
0
votes
0
answers
293
views
0
answers
... This one seems to be in the VCF 4.2 standard:
`##INFO=`
But as you say, the software using it may not be following the rules. Usually something like `-1` would be used to mean "no information" or "error". ...
written 17 months ago by
Torst • 960
0
votes
2
answers
529
views
2
answers
Comment:
C: awk extract certain line
... In Linux `dos2unix excel.txt` will fix the Windows line endings to be Unix compatible. ...
written 17 months ago by
Torst • 960
2
votes
1
answer
439
views
1
answers
... If your consensus accuracy is 99.9% then you still have 1 errors every 1000 bp. A typical bacterial gene is ~ 1000bp long. That 1 error is usually an indel. This results in a frame-shift in your CDS. If you use a gene finder like Prodgial (used in prokka) then you will get ~2 predicted CDS for eve ...
written 17 months ago by
Torst • 960
1
vote
0
answers
830
views
0
answers
... What minimum contig size did you include in that plot? ...
written 17 months ago by
Torst • 960
2
votes
2
answers
147
views
2
answers
... Spades often has trouble with 250bp PE reads.
I wrote Shovill to resolve this problem: https://github.com/tseemann/shovill
In your case I think however you have 1000s of TINY contigs.
You need to remove all the small contigs first to fix the N50 statistic.
`seqtk seq -L 500 contigs.fa > good_cont ...
written 17 months ago by
Torst • 960
0
votes
0
answers
1.3k
views
0
answers
... This fails: rm -fr foo && ./minia -in ~/tmp/R1.fastq -out-dir foo
This works: mkdir foo && ./minia -in ~/tmp/R1.fastq -out-dir foo
If 'foo' doesn't exist it spits LOTS of messages like this:
HDF5-DIAG: Error detected in HDF5 (1.8.11) thread 0:
#000: /home/vagrant/gatb-tools/gat ...
written 5.1 years ago by
Torst • 960
0
votes
1
answer
1.3k
views
1
answer
... Is this some issue with assumption of hdf5 library being installed at system level instead of using bundled one?
cd /tmp/minia20151207-2659-lh8xo7/minia-2.0.3-Source/build/ext/gatb-core/src && /bio/linuxbrew/bin/g++-5 -Os -w -pipe -march=core2 -I/tmp/minia20151207-2659-lh8xo7/minia-2.0.3-So ...
written 5.1 years ago by
Torst • 960
• updated
5.1 years ago by
Rayan Chikhi • 1.5k
0
votes
1
answer
2.3k
views
1
answers
Answer:
A: net charge of my protein
... EMBOSS has a "pepstats" tool to do this:
http://emboss.bioinformatics.nl/cgi-bin/emboss/help/pepstats
You can do it online here:
http://emboss.bioinformatics.nl/cgi-bin/emboss/pepstats
...
written 6.5 years ago by
Torst • 960
6
votes
1
answer
6.5k
views
1
answers
... A 6-mer is a subsequence of 6 letters (nucleotides or amino-acids).
For example, a length 10 DNA sequence has 5 possible 6-mers: the sequences using bases 1..6, 2..7, 3..8, 4..9, and 5..10. Think of a sliding window of length 6 moving across the sequence one letter at a time.
Aligning two sequenc ...
written 6.5 years ago by
Torst • 960
Latest awards to Torst
Scholar
17 months ago,
created an answer that has been accepted.
For A: New To Bioinformatics, What Is A 6-Mer When It Comes To Alignment Algorithms (An
Guru
17 months ago,
received more than 100 upvotes.
Popular Question
2.2 years ago,
created a question with more than 1,000 views.
For Samtools / Bcftools Missing Random Obvious Single Base Indels
Popular Question
2.2 years ago,
created a question with more than 1,000 views.
For minia 2.0.3 problem building from source - hdf5/hdf5.h missing?
Teacher
2.2 years ago,
created an answer with at least 3 up-votes.
For A: New To Bioinformatics, What Is A 6-Mer When It Comes To Alignment Algorithms (An
Appreciated
4.3 years ago,
created a post with more than 5 votes.
For A: New To Bioinformatics, What Is A 6-Mer When It Comes To Alignment Algorithms (An
Good Answer
4.3 years ago,
created an answer that was upvoted at least 5 times.
For A: New To Bioinformatics, What Is A 6-Mer When It Comes To Alignment Algorithms (An
Scholar
4.3 years ago,
created an answer that has been accepted.
For A: New To Bioinformatics, What Is A 6-Mer When It Comes To Alignment Algorithms (An
Scholar
4.3 years ago,
created an answer that has been accepted.
For A: Bacterial Genomes To Download
Popular Question
5.1 years ago,
created a question with more than 1,000 views.
For Samtools / Bcftools Missing Random Obvious Single Base Indels
Good Answer
5.9 years ago,
created an answer that was upvoted at least 5 times.
For A: Blast Can'T Find Databases
Good Answer
5.9 years ago,
created an answer that was upvoted at least 5 times.
For A: How To Blast A Sequence Against Multiple Databases
Appreciated
6.4 years ago,
created a post with more than 5 votes.
For A: How To Blast A Nucleotide Profile (Pssm) Against A Nucleotide Database?
Teacher
6.4 years ago,
created an answer with at least 3 up-votes.
For A: K-Mer Correction In Rna-Seq Data For Transcriptome Assembly?
Supporter
6.7 years ago,
voted at least 25 times.
Student
7.2 years ago,
asked a question with at least 3 up-votes.
For Samtools / Bcftools Missing Random Obvious Single Base Indels
Teacher
7.3 years ago,
created an answer with at least 3 up-votes.
For A: How To Blast A Nucleotide Profile (Pssm) Against A Nucleotide Database?
Teacher
7.8 years ago,
created an answer with at least 3 up-votes.
For A: Visualization Of Sequencing Data From Bacteria (Prokaryotes)
Teacher
9.1 years ago,
created an answer with at least 3 up-votes.
For A: How To Blast A Sequence Against Multiple Databases
Teacher
9.2 years ago,
created an answer with at least 3 up-votes.
For A: Blast Can'T Find Databases
Teacher
9.2 years ago,
created an answer with at least 3 up-votes.
For A: K-Mer Correction In Rna-Seq Data For Transcriptome Assembly?
Use of this site constitutes acceptance of our User
Agreement
and Privacy
Policy.
Powered by Biostar
version 2.3.0
Traffic: 1138 users visited in the last hour