User: nicholas.owen1

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Posts by nicholas.owen1

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RNA-seq subsampling for Monte Carlo simulation to test data?
... So I am investigating some RNA-seq data of two sample groups, each with 10+ samples, to look for differential gene expression and differential usage (DESeq2 and JunctionSeq respectively). From my understanding that the power of analysis is greater when I use all samples (as long as no outliers etc) ...
monte-carlo rna-seq written 3.7 years ago by nicholas.owen10
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Comment: C: RNA-SeQC memory error
... Thanks, I did and have no resolution as yet from there, but will follow up next week, will update :) ...
written 3.7 years ago by nicholas.owen10
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Comment: C: RNA-SeQC memory error
... Thanks for the advice, I have tried running BWA and everything is fine there, will look further into the memory allocated to java, thanks again ...
written 3.7 years ago by nicholas.owen10
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RNA-SeQC memory error
... Dear All, I have installed RNA-SeQC on our cluster and have been trying to run a number of jobs for RNA-SeQC (version 1.18) on 5-6 Gb BAM files of human RNA-seq data. Info about the BAM files: human paired-end sequences, aligned to hg38 build genome using STAR, read groups added, sorted and indexe ...
rna-seq written 3.8 years ago by nicholas.owen10 • updated 12 days ago by Biostar ♦♦ 20

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