User: eayasi

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eayasi0
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Posts by eayasi

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Comment: C: Using long reads to find a combination of mutations
... This is on the right track of what I want to do. I forgot to mention I am not working with a diploid genome, but a plasmid library. I am not sure if this program will give me the data I am looking for, but I will test it out. ...
written 2.4 years ago by eayasi0
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Using long reads to find a combination of mutations
... Does anyone know of a way to quantify multiple connected mutations on single reads? I am trying to use my nanopore reads to see if specific combinations stand out. The reads are pretty noisy and doing it by hand seems tedious! ...
long reads nanopore snp variants written 2.4 years ago by eayasi0 • updated 2.3 years ago by colindaven1.8k
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Comment: C: Including failed minION reads in alignment
... Thanks for your reply. I have had one read align in graphmap. :/ I have blasted some and gotten yeast mitochondrial DNA (makes sense since I isolated plasmid from yeast). Might have to rethink seq protocol to get usable data. ...
written 2.4 years ago by eayasi0
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Including failed minION reads in alignment
... I am trying to get data from a minION run I have done. Most of my reads a pretty low quality (rapid seq kit with shortened fragmentation on 8kb plasmid...might be the problem?) and over half failed. ![enter image description here][1] I've tried to use a few aligners like bwa-mem, graphmap, last - b ...
alignment minion written 2.4 years ago by eayasi0

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