User: sd.gamboa.t

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Posts by sd.gamboa.t

<prev • 6 results • page 1 of 1 • next >
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Comment: C: Data for drawing Heatmaps (RNA-seq)
... Hi Corentin, Thanks for your response. I had followed the trinity instructions and scripts to perform differential expression analysis (using the gene counts matrix). The trinity scripts also provided a mean to automatically perform several analysis, including a heatmap where the TMM matrix of dif ...
written 5 days ago by sd.gamboa.t0
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Data for drawing Heatmaps (RNA-seq)
... Hello, Please I'd like some advice.. I performed a de novo assembly (of RNA-seq reads) of the transcriptome of my target organism by means of Trinity. Next, I followed the Trinity pipeline and scripts to get the following data matrices about the assembled genes: - FPKM - TPM - TMM My question ...
fpkm heatmap tpm rna-seq tmm written 8 days ago by sd.gamboa.t0 • updated 8 days ago by Corentin50
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Comment: C: slect reference genes from transriptome data
... By comparing all the samples of the transcriptome, i want to select a couple of genes whose expression is stable among samples. Then, I intend to design primers based on such genes and use them as normalization reference for the qRT-PCR validation. ...
written 3 months ago by sd.gamboa.t0
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select reference genes from transriptome data
... Hi! I'm currently performing a transcriptome study. I got the differential expressed genes (DEG) from the data using edgeR. Now, I need to validate the transcriptome results by performing qRT-PCR of some of the transcripts. However, I'd like to select some reference genes from the transcriptome its ...
rna-seq written 3 months ago by sd.gamboa.t0 • updated 3 months ago by h.mon9.0k
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Comment: C: Resume Blast search
... Hi, the results file does exist. I'm applying your suggestion Thanks!! ...
written 11 months ago by sd.gamboa.t0
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Resume Blast search
... Hi everybody, I'm doing a blastx search with a stand-alone version in linux. My query file contains a big number of sequences (is a transcriptome assembly), so it takes some time to complete the analysis. For some reason (power goes out) at times the search is interrupted, so i need to start the ...
blast written 11 months ago by sd.gamboa.t0

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