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Posts by amelie.rouger
... Thank's for answering. Yes I used metagenome to map the RNA reads. I assembled metagenome with IDBA and annotated it with PROKKA. When I used available genome on ncbi to map the RNA reads I aligned only 30% of reads, that why we used metagenomes to map RNA reads. ...
... Hi everybody, I am trying to identified differentially expressed (DE) genes of several bacterial metatranscriptomes. To sum up the experimental plan. We have 2 bacterial communities (named E and U) inoculated under 3 different conditions A, B and C (reference condition). I collected both DNA and RN ...
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