User: maxwhjohn1988

gravatar for maxwhjohn1988
Reputation:
80
Status:
Trusted
Location:
Last seen:
16 hours ago
Joined:
2 years, 5 months ago
Email:
m************@gmail.com

Posts by maxwhjohn1988

<prev • 46 results • page 1 of 5 • next >
1
vote
1
answer
179
views
1
answers
Answer: A: RNAseq service recommendations
... I recently had a PE cDNA library constructed and sequenced by Eurofins (they're in Germany). They were cheap, relatively fast (although not incredible on that front), and the data were very high quality. For DNA library construction and sequencing I've used Novogene [for wgs/Illumina PE, 90 sample ...
written 8 weeks ago by maxwhjohn198880
2
votes
1
answer
2.8k
views
1
answers
Answer: A: unexpected bowtie2 unpaired alignment behavior
... Sorry to bump such a very old post, but I hit a similar problem a few months ago, which cost me a huge amount of time and tears thinking that my data were somehow to blame. If this is the same problem as I experienced (it looks similar), it's due to a bug in older versions of Bowtie2 which corrupts ...
written 12 weeks ago by maxwhjohn198880
1
vote
1
answer
123
views
1
answers
Answer: C: How to perform fst test using SNPs frequency?
... There are too many pieces of software available to do this for someone to choose one for you. You should go onto google and search for solutions. Try some, and come back to ask for help if you need help getting one of the available pieces of software to work. Some starting points would be PopGeno ...
written 3 months ago by maxwhjohn198880
0
votes
1
answer
123
views
1
answers
Comment: C: How to perform fst test using SNPs frequency?
... And is more or less identical to this from a few days before https://www.biostars.org/p/378106/ ...
written 3 months ago by maxwhjohn198880
0
votes
1
answer
123
views
1
answers
Comment: C: How to perform fst test using SNPs frequency?
... This is a duplicate of a post you made a few days ago https://www.biostars.org/p/379042/ ...
written 3 months ago by maxwhjohn198880
0
votes
0
answers
354
views
0
answers
Comment: C: Help scaffolding an Illumina genome assembly with 10x reads
... I've found a paper which goes into considerable detail with examples on how they ran Scaff10x. Section 3.2. I'm going to give this a try and will report back. [https://przeworskilab.com/wp-content/uploads/acropora-millepora-assembly.pdf][1] [1]: https://przeworskilab.com/wp-content/uploads/ac ...
written 4 months ago by maxwhjohn198880
0
votes
0
answers
603
views
0
answers
Comment: C: Enhancing draft genome using 10X data
... The last release was 8 months ago... ...
written 4 months ago by maxwhjohn198880
0
votes
0
answers
603
views
0
answers
Comment: C: Enhancing draft genome using 10X data
... Thanks for the suggestion, I'll take a look. The documentation is my issue, though, it's so poor that I don't really understand what to do or even where to start. ...
written 4 months ago by maxwhjohn198880
0
votes
0
answers
603
views
0
answers
Comment: C: Enhancing draft genome using 10X data
... Hi harish Thanks so much for replying. I'm using the newest version of Scaff10x. I haven't tried reading the gzipped fastq files via zcat, but I've had no issues running the program "normally" with gzipped fastq files - the program runs and exits cleanly, but just doesn't do anything (n contigs i ...
written 4 months ago by maxwhjohn198880
0
votes
0
answers
603
views
0
answers
Comment: C: Enhancing draft genome using 10X data
... Hi @harish Sorry to single you out like this but you're the only person I've encountered here who seems likely to be able to help. I've had no luck with Scaff10x, and I've made a post asking for help. Posting it here firstly in the hope that you see it and have something to say, and secondly in c ...
written 4 months ago by maxwhjohn198880

Latest awards to maxwhjohn1988

Supporter 4 months ago, voted at least 25 times.
Popular Question 6 months ago, created a question with more than 1,000 views. For Discovar De Novo assembly - "Attempt to allocate memory failed"
Scholar 22 months ago, created an answer that has been accepted. For A: Discovar De Novo assembly - "Attempt to allocate memory failed"
Teacher 22 months ago, created an answer with at least 3 up-votes. For A: DiscovarDenovo - fastq files should be interlaced
Scholar 23 months ago, created an answer that has been accepted. For A: Discovar De Novo assembly - "Attempt to allocate memory failed"

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1479 users visited in the last hour