User: pablo61991

gravatar for pablo61991
pablo6199130
Reputation:
30
Status:
New User
Location:
Last seen:
3 hours ago
Joined:
8 months, 1 week ago
Email:
p*********@gmail.com

Posts by pablo61991

<prev • 31 results • page 1 of 4 • next >
0
votes
0
answers
121
views
0
answers
Comment: C: RNAseq differential gene expression analysis yield results which come from diges
... You have find my first issue. I'm in the middle of my PhD, I started without so much idea about this technology and I have trusted in my supervisor. As you said, nowadays I can understand the misleading to work with a complete organism... The only explanation which I received to that design is just ...
written 6 days ago by pablo6199130
0
votes
0
answers
121
views
0
answers
Comment: C: RNAseq differential gene expression analysis yield results which come from diges
... Oh sorry I have forgotten to explain better the sampling. We are working with a very little stage, we can call it larvae. As consequence, we are working with a complete individual. So I have my mRNA coming from my individual (larva of one cephalopod species). The result of sequence that were a bunc ...
written 7 days ago by pablo6199130
0
votes
0
answers
121
views
0
answers
RNAseq differential gene expression analysis yield results which come from digestive content
... Hi Community, I have done my transcriptome assembly, my annotation and also my differential gene expression analysis (kallisto to get pseudocounts, tximport to give them to DESeq2, and DESeq2 to test differential expression). Now when I'm trying to work with some of my extremely up-regulated gene ...
0
votes
2
answers
169
views
2
answers
Comment: C: DGE on transcriptomic data, focus on transcript sequences or in derivated ORFs?
... Thank you for your explanation, I'll try to apply the analysis in this way. ...
written 27 days ago by pablo6199130
1
vote
2
answers
169
views
2
answers
DGE on transcriptomic data, focus on transcript sequences or in derivated ORFs?
... Hi Community, I have the next doubt, if I'm only interested in protein coding genes it's a good practice to obtain the ORFs from my transcriptome (de novo assembled) using for example Transdecoder or EMBOSS tools and then quantify the levels of expression?? The pipeline could be something like: - ...
orfs rna-seq differential gene expression written 4 weeks ago by pablo6199130 • updated 4 weeks ago by gilbert.bionet110
0
votes
0
answers
147
views
0
answers
Comment: C: Doubts using Velvet (transcriptome assembly) mainly in performance
... Something wrong in my way to question or just a complicated problem? ...
written 4 weeks ago by pablo6199130
0
votes
0
answers
147
views
0
answers
Doubts using Velvet (transcriptome assembly) mainly in performance
... Hello community I'm running Velvet right now and I have several doubts because I'm in memory issues. I have tried to solve that but I wasn't successful. I'm novice using this software (and I'm not bioinformatician). To contextualize, I'm following the protocol uploaded [here][1]. I found it pretty ...
de novo assembly velvet rna-seq transcriptome written 4 weeks ago by pablo6199130 • updated 4 weeks ago by genomax39k
0
votes
1
answer
245
views
1
answers
Comment: C: primer desing for qPCR on de novo assembly output
... Thank you Kevin, I have more success using your tips ;) ...
written 10 weeks ago by pablo6199130
0
votes
1
answer
245
views
1
answers
Comment: C: primer desing for qPCR on de novo assembly output
... Sorry but is something wrong? I can see a lot of views but no answers or comments, there is something wrong in my statement? ...
written 4 months ago by pablo6199130
2
votes
1
answer
245
views
1
answer
primer desing for qPCR on de novo assembly output
... Hello community, I have searched in forums and google looking for help in primer design for confirmation assay by qPCR of de novo assembly transcripts. When I blast my transcripts, I have obtained different levels of identity against the blast hit and when I have to design my primers I don´t know i ...
rnaseq primer qpcr written 4 months ago by pablo6199130 • updated 3 months ago by Kevin Blighe9.3k

Latest awards to pablo61991

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1167 users visited in the last hour