User: charlesgwellem

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Posts by charlesgwellem

<prev • 6 results • page 1 of 1 • next >
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Seurat: Percentage distribution of cells in all clusters based on their treatment condition?
... Is there are possibilty in Seurat to visualise the percentage distributions of cells treated under a particular condition per cluster? For example I wish to be able to produce a figure where I say: X% of cells treated with Y condition were located in cluster C and so forth? I have tried: numbe ...
R rna-seq seurat single cell written 14 days ago by charlesgwellem0 • updated 14 days ago by Santosh Anand4.6k
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Data base or computational tool to help predict if two predicted interacting surface proteins are located on separate cells or on the same cell?
... Is there any computational method or data base with the help of which one could predict if two interacting cell surface proteins are located on separate cells or are located on the same cell surface? ...
genome bioinformatics rna-seq written 22 days ago by charlesgwellem0
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Given a list of cell surface genes, is there a computational possibility to predict their interaction partners on the surface of another cell?
... Hi folks. After performing differential expression analyses on my single cell RNA sequencing data from ILC2s of mouse origin, treated under three conditions, I generated a list of differentially genes. I afterwards used the Gene Ontology platform to generate a shorter list of genes proven to be pres ...
gene R rna-seq fibroblasts geneontology written 27 days ago by charlesgwellem0 • updated 26 days ago by kristoffer.vittingseerup1.4k
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How do I identify which differentially expressed genes are on the cell surface?
... I am very new to single cell sequencing and IPA analyses, but I have a background in R and Python programming. I have a set of differentially expressed genes from the single cell sequencing data of a cell type cultured alone under three conditions including the control condition. I have made use of ...
R sequencing surfacemarkers ipa python written 28 days ago by charlesgwellem0 • updated 28 days ago by Jean-Karim Heriche18k
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(Closed) Why this change in morphology in endothelial colony forming cells (ECFC)?
... Dear colleagues, I culture on 2% PAN-biotech gelatine, cord blood MNCs into endothelial colony forming cells (ECFCs), using Endopan 3 kit medium.  For many of my samples, I obtain a change in morphology of my ECFCs during culture, and these "new looking"  ECFCs no longer proliferate. The cells now ...
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(Closed) Why this change in morphology in endothelial colony forming cells (ECFC)?
... Dear colleagues, I culture on 2% PAN-biotech gelatine, cord blood MNCs into endothelial colony forming cells (ECFCs), using Endopan 3 kit medium.  For many of my samples, I obtain a change in morphology of my ECFCs during culture, and these "new looking"  ECFCs no longer proliferate. The cells now ...

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