User: charlesgwellem

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Posts by charlesgwellem

<prev • 10 results • page 1 of 1 • next >
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Comment: C: Simple user-friendly tool to find the potential binding site of a particular DN
... Ok thanks saw it. I wish to find out about the existence of a simple user-friendly online tool, or an R package rather than methods that are more computationally intensive. ...
written 11 weeks ago by charlesgwellem0
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Can HOMER be used to predict the binding site of a transcription factor on a DNA sequence?
... I wish to find out if HOMER tool can be used to predict the binding site of a transcription factor on a list of DNA sequences. Thank you in advance for your kind and helpful responses. ...
genome gene chip-seq rna-seq homer written 11 weeks ago by charlesgwellem0 • updated 11 weeks ago by Alex Reynolds28k
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Comment: A: Is there a data base, tool or method I can use to find out which of my genes cod
... Thank you very múch. I searched the [GO term data base][1] for "cytokine receptor activity" and I got [this][2] which consists of a set of genes which I can download as an excel file. My next step will be to programmatically match the genes in the excel file to those in my gene list and subset from ...
written 5 months ago by charlesgwellem0
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Is there a data base, tool or method I can use to find out which of my genes code for cytokine receptors?
... I have a list of over 600 differentially expressed genes from my single cell RNA seq data analyses. I want to proceed to find out which of my genes code for cytokine receptors so that I can show on a heat map how their expression varies across clusters. Can any one give me a hint on which tool o ...
gene genome rna-seq R written 5 months ago by charlesgwellem0
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Seurat: Percentage distribution of cells in all clusters based on their treatment condition?
... Is there are possibilty in Seurat to visualise the percentage distributions of cells treated under a particular condition per cluster? For example I wish to be able to produce a figure where I say: X% of cells treated with Y condition were located in cluster C and so forth? I have tried: numbe ...
R rna-seq seurat single cell written 6 months ago by charlesgwellem0 • updated 6 months ago by Santosh Anand4.9k
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Data base or computational tool to help predict if two predicted interacting surface proteins are located on separate cells or on the same cell?
... Is there any computational method or data base with the help of which one could predict if two interacting cell surface proteins are located on separate cells or are located on the same cell surface? ...
genome bioinformatics rna-seq written 6 months ago by charlesgwellem0
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Given a list of cell surface genes, is there a computational possibility to predict their interaction partners on the surface of another cell?
... Hi folks. After performing differential expression analyses on my single cell RNA sequencing data from ILC2s of mouse origin, treated under three conditions, I generated a list of differentially genes. I afterwards used the Gene Ontology platform to generate a shorter list of genes proven to be pres ...
gene R rna-seq fibroblasts geneontology written 6 months ago by charlesgwellem0 • updated 6 months ago by kristoffer.vittingseerup2.2k
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How do I identify which differentially expressed genes are on the cell surface?
... I am very new to single cell sequencing and IPA analyses, but I have a background in R and Python programming. I have a set of differentially expressed genes from the single cell sequencing data of a cell type cultured alone under three conditions including the control condition. I have made use of ...
R sequencing surfacemarkers ipa python written 6 months ago by charlesgwellem0 • updated 6 months ago by Jean-Karim Heriche20k
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(Closed) Why this change in morphology in endothelial colony forming cells (ECFC)?
... Dear colleagues, I culture on 2% PAN-biotech gelatine, cord blood MNCs into endothelial colony forming cells (ECFCs), using Endopan 3 kit medium.  For many of my samples, I obtain a change in morphology of my ECFCs during culture, and these "new looking"  ECFCs no longer proliferate. The cells now ...
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(Closed) Why this change in morphology in endothelial colony forming cells (ECFC)?
... Dear colleagues, I culture on 2% PAN-biotech gelatine, cord blood MNCs into endothelial colony forming cells (ECFCs), using Endopan 3 kit medium.  For many of my samples, I obtain a change in morphology of my ECFCs during culture, and these "new looking"  ECFCs no longer proliferate. The cells now ...

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