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Posts by zaidnab
... Hi there! I am trying to edit values in a bam file, using Pysam, or any other interface. I want to change the base quality, and the read sequence of certain reads. I have searched a ton, but have not found any methods of editing bam files directly. I am not sure if this is possible. Any help would b ...
... I am trying to write a python script that, from a BAM files, calculates the error rate of the DNA sequencing based on the reference genome. I am brand new to bioinformatics, and I am very stuck. This is what I have so far: import pysam samfile = pysam.AlignmentFile("TruQ3_229.sorted.bam", "rb") ...
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