User: Hughie

gravatar for Hughie
Hughie10
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10
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New User
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Last seen:
12 hours ago
Joined:
1 year, 7 months ago
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h*********@gmail.com

Posts by Hughie

<prev • 18 results • page 1 of 2 • next >
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Comment: C: Split fastq according to barcodes
... It's 10 times faster than my previous scripts and it only toke ~1h to split my 40G gzipped PE data! So, amazing! Thank you again :) ...
written 4 weeks ago by Hughie10
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Comment: C: Split fastq according to barcodes
... Thanks a lot for your reply fin swimmer! This solution is so amazing, and it's what I'm looking for. I have tested it on my server and I will report the running time later. By the way, linux command is so powerful and also complicated which I have a long way to go... ...
written 4 weeks ago by Hughie10
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Comment: C: Split fastq according to barcodes
... Thanks cpad0112 for your reply, I have tried this method before but it can only handle SE data, and I have to get a script for pairing read1 and read2. ...
written 4 weeks ago by Hughie10
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Comment: C: Split fastq according to barcodes
... Thanks for your reply, genomax , but this protocol is modified, so the barcode is in the head 8bp of Read_2. At the very beginning when I recieved the data, I also thought the barcode is in the header of both reads, so weird a protocol :( ...
written 4 weeks ago by Hughie10
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Comment: C: Split fatsq according to barcodes
... The first version I wrote is too slow, and I found a python script [paired-end_barcode_splitter][1] from github. It uses a perl script named **"fastx_barcode_splitter.pl"** from FASTX-toolkit to split my read_2 data according to barcode information and after that, fastq header was used to match th ...
written 4 weeks ago by Hughie10
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Comment: C: Split fatsq according to barcodes
... No, it's in the head 8bp of read_2 ...
written 4 weeks ago by Hughie10
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Split fastq according to barcodes
... Hello, everyone: I'm recently analyze my scRNA-seq data, the first step is to splitting fastq files according to my barcode file which looks like this: sc1 AACGTGAT sc2 AAACATCG sc3 ATGCCTAA sc4 AGTGGTCA sc5 ACCACTGT sc6 ACATTGGC sc7 CAGATCTG sc8 CATCAAGT sc ...
scrna-seq fastq demultiplexing written 4 weeks ago by Hughie10 • updated 4 weeks ago by finswimmer8.9k
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Forum: Freshman puzzled by tons of statistic methods
... Hello everyone! I'm a freshman of bioinformatics and I'm at first year of master degree, I got a lot of troubles when learning. 1. I want to ask whether I need to know every statistic methods when using a new packages? although it seems important when fune-tune the parameters, I got in trouble ...
biostatistics forum ml statistics written 3 months ago by Hughie10 • updated 3 months ago by WouterDeCoster35k
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Comment: C: awk specific line from another file
... Thank you ! Revised again! ...
written 6 months ago by Hughie10
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Comment: C: awk specific line from another file
... Thank you 5heikki! This is a new solution ...
written 6 months ago by Hughie10

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Supporter 4 weeks ago, voted at least 25 times.

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