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Posts by k.mooo
... I am performing RNA-seq analysis using the following pipe - HISAT2 - StringTie - Ballgown I used a cell-type specific enrichment method to target reads. I performed qRT with this same sample to quantify the presence of a single transcript and saw enrichment for this transcript. For those intereste ...
... 0 expression values could indicate an incongruous reference genome between alignment and annotation. The same version, as well as the same source (for example, Ensembl or UCSC), must be used when aligning reads and annotating reads. This is mentioned at the bottom of this blog with respect to refer ...
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