User: a.alsayed.phd

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Posts by a.alsayed.phd

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Comment: C: species relative abundance
... Thank you for your advise I know, but I have around 137 rows, 3 examples (OTU ID) as following: k__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus k__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Actinomycetales;f__Micrococcaceae;g__Rothia k__Bacteria;p__ ...
written 9 days ago by a.alsayed.phd0
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Comment: C: species relative abundance
... Thank you for your reply I laready know the abundance for each OTU and I am trying to know the relative abundance and plot it. ...
written 10 days ago by a.alsayed.phd0
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species relative abundance
... Hello, I have OTUs (from 16 s microbiome analysis - MIseq) on excel sheets for many samples. I would like to plot the relative abundance of each species in a graph. I am new to this field, so please, can anyone advise me how to do that? Many Thanks ...
16s written 10 days ago by a.alsayed.phd0 • updated 10 days ago by Brian Bushnell14k
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Answer: A: reference genome alignment
... MSA using DNASTAR lasergene software is one option ...
written 12 days ago by a.alsayed.phd0
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Comment: C: sense versus antisense (plus or minus) sequence alignment
... DNA seqs reads - sanger sequencing of PCR products viral capsid regions (VPs) of human rhinoviruses ...
written 12 days ago by a.alsayed.phd0
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sense versus antisense (plus or minus) sequence alignment
... Please, if I have a couple of query (parts of virus genome) sequences and a couple of references sequences, what are the steps required to align them correctly;i.e, to ensure that I alighn sense with sense etc. (the same direction)? Many Thanks ...
assembly sequence blast alignment sequencing written 18 days ago by a.alsayed.phd0
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Minimum numbers of sequence bases in Sanger sequencing
... If I am working on Sanger sequencing results for a certain type of viruses, by assays that target parts of the genome, so I am using few assays for each sample, got a couple of sequencing reads for each sample. Then, assembly, MSA, then phylogenetics: Please, is there any recommended minimum sequenc ...
genome assembly alignment sequence sequencing written 22 days ago by a.alsayed.phd0

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