User: bioxc

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Posts by bioxc

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Can I use strand-specific RNA-seq data as non-stranded data when aligning them by some software (e.g. tophat, using fr-unstranded) if I just want to calculate the gene expression level?
... Can I use strand-specific RNA-seq data as non-stranded data when aligning them by some software (e.g. tophat, using fr-unstranded) if I just want to calculate the gene expression level? If so, how does it affect the result except for losing the strand information, e.g. the gene expression calculatio ...
alignment written 8 weeks ago by bioxc0 • updated 8 weeks ago by Devon Ryan71k
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Comment: C: how to measure gene expression level using third sequencing long reads (for exam
... Thanks! Why can't ISO-seq be quantitative? The main difference between ISO-seq and illumina RNA-seq is the reads length, so the sequencing results should be the number of reads of each isoform, if so, why don't we normalize them by the library total reads to estimate the gene expression level, like ...
written 9 weeks ago by bioxc0
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Comment: C: how to measure gene expression level using third sequencing long reads (for exam
... But what is the purpose of the ISO-seq of PacBio? It is for transcriptomics. ...
written 9 weeks ago by bioxc0
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Comment: C: how to measure gene expression level using third sequencing long reads (for exam
... Thanks, could you explain to me more about the non-quantification of PacBio sequencing? ...
written 9 weeks ago by bioxc0
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how to measure gene expression level using third sequencing long reads (for example, PacBio long reads)
... When I read some papers which have used third sequencing technology, e.g. PacBio iso-seq, I didn't find any paper which use the long reads to measure gene expression level. They did RNA-seq by third sequencing, but measured gene expression level by second sequencing short reads. Can someone tell me ...
gene rna-seq sequencing written 9 weeks ago by bioxc0 • updated 9 weeks ago by geek_y8.0k

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