User: xqyjxau

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xqyjxau40
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2 years, 8 months ago
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Posts by xqyjxau

<prev • 5 results • page 1 of 1 • next >
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Relationship of TCGA, Firehose and cBioportal
... I'm confused about the relationship of TCGA, Firehose and cBioportal data. Are the data from each database are totally different, or partly/largely overlapping? Some links or references? ...
genome next-gen written 12 months ago by xqyjxau40 • updated 12 months ago by i.sudbery7.0k
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Comment: C: Extremely low assigned reads in featureCounts
... No.It's not strand-specific. ...
written 2.7 years ago by xqyjxau40
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7 follow
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Extremely low assigned reads in featureCounts
... Hi!Talents! Here are my question:I used my RNA-Seq data(fastq format of human sample). I chose the GRCh38 and GRCh38.89.gtf for STAR reference genome indexing. And later I use featureCounts to count the reads(same gtf file of GRCh38.89.gtf). Here is my command: featureCounts -T 5 -a -t exon - ...
genome sequence alignment rna-seq written 2.7 years ago by xqyjxau40 • updated 2.7 years ago by Istvan Albert ♦♦ 82k
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Comment: C: SAM file size after STAR alignment
... The data uniquely mapped is from 63%-64%, multiply mapped reads are from 27% to 32%.Is this OK? ...
written 2.7 years ago by xqyjxau40
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SAM file size after STAR alignment
... My RNA-Seq data is in format of fastq(ungzipped from fastq.gz format).I used STAR 2.5.3a mapping the reads with already indexed reference genome. It seems good. But I found the size of generated SAM file is strange. My original input fastq data is like 1.3-1.5 GB, but the SAM file ranges from 3.8 GB ...
alignment rna-seq written 2.7 years ago by xqyjxau40 • updated 2.7 years ago by Istvan Albert ♦♦ 82k

Latest awards to xqyjxau

Popular Question 20 months ago, created a question with more than 1,000 views. For Extremely low assigned reads in featureCounts
Popular Question 20 months ago, created a question with more than 1,000 views. For SAM file size after STAR alignment

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