User: hamaor

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hamaor0
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Posts by hamaor

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Answer: A: High no feature counting when using strand specific data
... I tried it. counting percentage drops dramatically. though samples has high percentage of "no feature" when strand= yes presents more counted reads than others when strand = reverse. ...
written 4 months ago by hamaor0
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High no feature counting when using strand specific data
... As i'm analyzing strand-specific RNA-seq data, prepared with Epicenter kit, I used HT-Seq count with the option: -s yes Some of my samples had only ~50% counted reads (the rest is had no feature) while others over 85%. i tried to count again, this time -s no, than all of my samples had over 85% coun ...
count strand specific epicentre rna-seq written 4 months ago by hamaor0
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Comment: C: help with Gene Bank annotation and GFF file
... that was extremely helpfull!! i appriciate it! ...
written 4 months ago by hamaor0
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help with Gene Bank annotation and GFF file
... Hi everyone, I downloaded one of E.coli's strains fasta file from [here][1]. unfortunately i couldn't find its compatible gff file. in this point if any one can help me find one it could be awsome! I wrote a script converting fasta to gff3 using gene features fasta file found in the same link. most ...
gff genebank rna-seq written 4 months ago by hamaor0 • updated 4 months ago by h.mon9.7k

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