User: lucilepain

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lucilepain0
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Posts by lucilepain

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Comment: A: Online course: Learn Bioinformatics in 100 hours
... Hi, I am processing the lecture 1 until running the doctor.py, which doesn't work :/ Here is what my terminal shows: bash: /root/bin/doctor.py: /usr/bin/python: bad interpreter: No such file or directory So I did: "ls" which gave "Miniconda3-latest-Linux-x86_64.sh bash_profile bin ...
written 24 months ago by lucilepain0
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Comment: C: Calculate forward and reverse strand reads from bam
... Hello everyone, I am trying to process to counting from data "forward.bam" and "reverse.bam" that have been previously mapped (paired-end mode). I would like to use HT-seq counts , that takes only one .bam as input (forward+reverse). Does anyone know about some tools or documentations that allow to ...
written 24 months ago by lucilepain0
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Comment: C: Counting number of genes using htseq-count tool.
... Hello Leipinji, then do you know how to reunite .forward.bam and .reverse.bam, previously mapped, together to process to counting ? Many thanks ...
written 24 months ago by lucilepain0
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Comment: A: Forward Stand Or Reverse Strand
... Hello everyone, This post is really interesting, I actually need to analyse (ht-seq counts) .bam files (sorted and indexed) that have been mapped by someone else before me. My files are as this form: - sample.merged.mdup.forward.bam - sample.merged.mdup.reverse.bam. When looking for ht-seq counts ...
written 24 months ago by lucilepain0
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Comment: C: Help: RNA-seq analysis
... Thanks, i already read that page but what remained unclear to me, was the use of files .bed and .bai if , according to the page, only my .bam are required. Any idea? Thank you very much for your help. ...
written 2.1 years ago by lucilepain0
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Help: RNA-seq analysis
... Hello everyone, My post will probably look "basic" to many of you but either, let's go. I am really beginner (I have the foundations of the modules available via Coursera) so my terminology may be incorrect. I have to analyze RNA-seq data in order to arrive at the top differentially expressed gene ...
rna-seq written 2.1 years ago by lucilepain0 • updated 2.1 years ago by mforde841.2k

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