User: jkbonfield
jkbonfield • 70
- Reputation:
- 70
- Status:
- New User
- Location:
- Last seen:
- 2 days, 19 hours ago
- Joined:
- 1 year, 10 months ago
- Email:
- j*********@gmail.com
Posts by jkbonfield
<prev
• 4 results •
page 1 of 1 •
next >
3
votes
1
answer
175
views
1
answers
... Firstly, don't mount those drives read-write any more. Read-only from now on or you'll exacerbate any data recovery chance by either yourself or professionals.
BAM has specific signatures that data recovery tools are unlikely to spot, but you could, *perhaps* extract them yourself from raw disk im ...
written 2 days ago by
jkbonfield • 70
1
vote
2
answers
1.3k
views
2
answers
... There is samtools addreplacerg which adds or replaces RG tags in records too, but it is a fixed string rather than derived per barcode. This may be useful if you already have files split up per barcode, but not otherwise.
If they're all mixed together, then you'll need to read in a table and do a ...
written 12 months ago by
jkbonfield • 70
2
votes
2
answers
692
views
2
answers
... In *SAM* format the field is '\*'. In *BAM* format this is replaced by a run of 0xff values to match the same length as the sequence. (I've no idea why.)
Note this raises a curious ambiguity. How does a single base read with confidence 9 ('*') get interpreted? ...
written 22 months ago by
jkbonfield • 70
1
vote
1
answer
936
views
1
answers
... SA:Z: tags appear on the primary reads as well as supplementary ones, therefore the count is higher. Filtering by flags is the best approach. (Hint "samtools flags" give you a handy chart of the numbers.) ...
written 22 months ago by
jkbonfield • 70
Latest awards to jkbonfield
No awards yet. Soon to come :-)
Use of this site constitutes acceptance of our User
Agreement
and Privacy
Policy.
Powered by Biostar
version 2.3.0
Traffic: 562 users visited in the last hour