User: 11yj3312

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11yj33120
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Posts by 11yj3312

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crispr mageck analysis ,Sam library
... Dear all, I download SAM Library from website,but there is only guide sequence and gene-number,like this: NM_000014_1 AAGTGAGCTCTTACGGGAAT NM_000014 NM_000014_2 GAATGTAGTTTTAGCCCTCC NM_000014 NM_000014_3 GGGATTCTATTTAGCCCGCC NM_000014 how can i kown the gene_id of ea ...
genome crispr sam library written 2 days ago by 11yj33120 • updated 2 days ago by Pierre Lindenbaum102k
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Comment: C: Question: Error HISAT2 output for HTseq-count
... I have tried the latest htseq-count,No previous error occurred.Think you! ...
written 2 days ago by 11yj33120
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Comment: C: Question: Error HISAT2 output for HTseq-count
... Think you !I will try it ...
written 3 days ago by 11yj33120
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Question: Error HISAT2 output for HTseq-count
... Dear all, I am analyzing paired-end stranded RNAseq data . I would be interested in using HISAT2 for the alignment, and HTseq-count to count the features of the aligned reads. When I used HTseq-count to count the reads: htseq-count -r pos -f bam SRR3589957_sorted.bam ../reference/gencode.v26lift37. ...
software error alignment rna-seq written 3 days ago by 11yj33120
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Mageck pathway command
... I obtain a pathway summary by using mageck pathway command,as follows: id num neg|score neg|p-value neg|fdr neg|rank neg|goodsgrna pos|score pos|p-value pos|fdr pos|rank pos|goodsgrna KEGG_RIBOSOME 87 8.3272e-23 2.6473e-05 0.001238 1 50 0 ...
software error gene sequence written 3 months ago by 11yj33120

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