User: Ambika

gravatar for Ambika
Ambika30
Reputation:
30
Status:
New User
Location:
United States/Auburn/Auburn University
Last seen:
6 days, 8 hours ago
Joined:
1 year, 10 months ago
Email:
a***************@gmail.com

Posts by Ambika

<prev • 94 results • page 2 of 10 • next >
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qPCR: Huge variation in fold change of genes between biological replicates
... Hello everyone, I am trying to validate my RNAseq data by doing qpcr for which I am looking at the fold change of few genes across various timepoints of treatment conditions. I am getting huge amount of variation (in thousand folds ) in my biological replicates. I thought may be it is due to genomi ...
foldchange qpcr written 13 months ago by Ambika30 • updated 13 months ago by WouterDeCoster40k
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Comment: C: Gene enrichment analysis
... Thank you I will try that ...
written 15 months ago by Ambika30
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Gene enrichment analysis
... Hello everyone,, I am working with RNAseq data from a fungi for which we don't have genome annotation. I have a draft genome and list of predicted genes from Augustus. From RNAseq I have list of genes for which I want to do enrichment analysis. I tried to use DAVID for which I tried to get UniProt ...
gene_enrichment david blast2go uniprot written 15 months ago by Ambika30 • updated 15 months ago by Buffo1.6k
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Comment: C: Enrichment analysis of Differentially expressed genes
... Hi, h.mon, I was looking on how can I associate each identified gene with UniProt identifier, but I am lost. Can you suggest a way I can do that. ...
written 15 months ago by Ambika30
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Comment: C: Enrichment analysis of Differentially expressed genes
... Yes you are right I have an assembled draft genome. Right now I am trying to blast my draft genome using Blast2Go and looks like it will take forever. I will try the other idea you said using UniProt. Thank you h.mon for helping. ...
written 15 months ago by Ambika30
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Comment: C: Enrichment analysis of Differentially expressed genes
... No it is not published and not annotated ...
written 15 months ago by Ambika30
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Enrichment analysis of Differentially expressed genes
... Hello everyone, I used STAR-HTseq-DESeq2 pipleline to analyse my RNAseq data and now I have list of differentially expressed genes. I want to do the enrichment analysis of the list of genes obtained from DEseq2. I tried to use DAVID but it didn't work for me since I am working with non-model organ ...
rna-seq enrichment analysis written 15 months ago by Ambika30
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Comment: C: arranging columns and rows
... Thank you so much for your help. ...
written 15 months ago by Ambika30
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Comment: C: arranging columns and rows
... Hi Pierre, Thank you but the problem is I got the output file but the number of rows that I have for output file is not the same as in File 2. ...
written 15 months ago by Ambika30
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Comment: C: arranging columns and rows
... Yes its present twice, and this is just a sample some of the genes might be present more than that because single gene might have different pfam domains. ...
written 15 months ago by Ambika30

Latest awards to Ambika

Supporter 10 days ago, voted at least 25 times.
Popular Question 13 months ago, created a question with more than 1,000 views. For Deseq2 library load error
Popular Question 13 months ago, created a question with more than 1,000 views. For Unable to access jarfile trimmomatic-0.35
Popular Question 13 months ago, created a question with more than 1,000 views. For HTseq Pysam error
Popular Question 13 months ago, created a question with more than 1,000 views. For Trimmomatic jar file
Popular Question 13 months ago, created a question with more than 1,000 views. For Interpretation of Results from trimmomatic
Popular Question 13 months ago, created a question with more than 1,000 views. For HTseq error: Too few arguements
Popular Question 13 months ago, created a question with more than 1,000 views. For DESeq2 workflow for RNAseq
Popular Question 13 months ago, created a question with more than 1,000 views. For Feature Counts output interpretation
Popular Question 15 months ago, created a question with more than 1,000 views. For Unable to access jarfile trimmomatic-0.35
Rising Star 20 months ago, created 50 posts within first three months of joining.

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