User: Claire

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Claire40
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Location:
Germany
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3 hours ago
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1 month, 1 week ago
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y**************@gmail.com

Posts by Claire

<prev • 27 results • page 1 of 3 • next >
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Comment: C: Differential gene expression based on "exon" or "CDS"?
... I am not sure "RNA is composed of exons" is true after reading [this paper][1]. I don't want to be rude. I just want to know whether my understanding is right. [1]: https://bmcbiol.biomedcentral.com/articles/10.1186/1741-7007-8-149 ...
written 6 days ago by Claire40
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Comment: C: Differential gene expression based on "exon" or "CDS"?
... I don't agree with what you said about differntial isform/CDS usage. Differential isoform expression (DIE) and differential isoform usage (DIU) are related but distinct concepts. DIE assesses the difference of absolute expression in isoform level. In contrast, DIU assesses the difference of relative ...
written 6 days ago by Claire40
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Comment: C: Differential gene expression based on "exon" or "CDS"?
... Sorry about the misunderstanding caused by my poor English, I am not meaning bad. What I mean when I say biology is too complex, is that when we analyze some biology problems, sometimes we have to do some compromise. For example, when we talk about measuring gene expression level, it is based on t ...
written 6 days ago by Claire40
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Comment: C: Differential gene expression based on "exon" or "CDS"?
... The biology is too complex... I know that the eukaryotic genomes are pervasively transcribed, and that the majority of its bases can be found in primary transcripts, including non-protein-coding transcripts, and many biological processes utilize and require noncoding RNAs. But when we talk about ...
written 7 days ago by Claire40
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Comment: C: Differential gene expression based on "exon" or "CDS"?
... Since in the annotation gtf file I see "exon" and "CDS". I thought edfferential expressed gene analysis could be based on CDS, which is more meaningful in my understanding ...
written 7 days ago by Claire40
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Comment: C: Differential gene expression based on "exon" or "CDS"?
... Since in the annotation gtf file I see "exon" and "CDS". chr1 unknown exon 3214482 3216968 …... chr1 unknown stop_codon 3216022 3216024 …... chr1 unknown CDS 3216025 3216968 …... chr1 unknown CDS 3421702 3421901 …... ...
written 7 days ago by Claire40
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Differential gene expression based on "exon" or "CDS"?
... Hi all, When we talk about differential gene expression analysis using RNA-seq data, what we actually evaluate is the expression of "exon" or "CDS"? ![enter image description here][1] [1]: https://i.imgur.com/NJmVWeZ.png ...
gene rna-seq written 7 days ago by Claire40 • updated 7 days ago by Devon Ryan71k
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Comment: C: detailed explanation of Insert Size
... Thank you for your advice, I will give it a try. ...
written 10 days ago by Claire40
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Comment: C: detailed explanation of Insert Size
... Thank you for your clear explanation! ...
written 10 days ago by Claire40
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Comment: C: detailed explanation of Insert Size
... Yes, this is included in the background of my question... My question is: First, in RNA seq data, if the alignments are spliced, and the TLEN reports the distance from the 5'-most to 3'-most position ([if my understanding is right][1]). So according to my understaning the TLEN number will include ...
written 10 days ago by Claire40

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