User: serenabivona

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serenabivona10
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Posts by serenabivona

<prev • 7 results • page 1 of 1 • next >
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Comment: C: CDS FASTA file as Reference sequence for Ion Torrent
... Hi Kevin! Sorry :)! I forgot to explain that I'm not interested to gene expression, my purpose is study, by ngs, mutations that occur in the tyrosine kinase domain (TKD) of abl gene, that is part of the fusion gene bcr-abl. To exclude the possibility to sequence the allele not trasclocated (wild-ty ...
written 21 months ago by serenabivona10
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Comment: C: CDS FASTA file as Reference sequence for Ion Torrent
... Thank you Titus.....please, could you explain it better? Thank you! Sere ...
written 21 months ago by serenabivona10
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Answer: A: CDS FASTA file as Reference sequence for Ion Torrent
... Thank you all for your answer! You were very helpful to clarify my question. So, I think that is usless or improper create a file.bed....because I read that the bed file needs for the track name chromosome, start and end position as minimal data, unless I could create a "similar bed file" with posi ...
written 21 months ago by serenabivona10
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Comment: C: CDS FASTA file as Reference sequence for Ion Torrent
... i.e. Can I use my reference file "cds" .fasta as reference instead of a classic reference genome hg19 to plun the run?? Thank you... ...
written 21 months ago by serenabivona10
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CDS FASTA file as Reference sequence for Ion Torrent
... Hi! I'm sorry! My question will be stupid for somone else, but it is my first approach with ngs analysis... I will use PGM and Torrent Suite Software to sequence a small region of interest. In summary, my sample is cDNA and my amplicons span only a part of a CDS of my human gene of interest, with ...
fasta cds .bed next-gen reference written 21 months ago by serenabivona10
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Comment: C: hg19 RNA info
... Thank you for your answer. However, if it is possible, I would need more info...for example, using Biomart I found again only the position of my exon on chromosome, but not the corresponding position on the entire trapscriptome, if they exist. Maybe I don't know where exactly search and what kind of ...
written 21 months ago by serenabivona10
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hg19 RNA info
... Hello! I need information about the Transcriptome file for hg19, hg38 or similar files that can help me in my purpose. In brief, I have the coordinates of exons from a cds of my interest (starts and end positions) on hg19 and hg38.... ex: Chr. 9 start 130883969 end 130885683 ....but for my applicat ...
gene next-gen alignment rna-seq written 21 months ago by serenabivona10 • updated 21 months ago by tarek.mohamed250

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