User: Silvia

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Silvia10
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Posts by Silvia

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Comment: C: FPKM or rlog from DEseq2 for machine learning analysis
... Upps, I thought I replied directly to you! :) Ok, I see the idea. Thank you very much for your help!! Silvia ...
written 15 months ago by Silvia10
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Comment: A: FPKM or rlog from DEseq2 for machine learning analysis
... thanks Kevin, I obtained the FPKM values with RSEM using the same count matrix that I use to calculate the rlog. Do you have any idea of why these values seem wrong? Also, I have plotted the same values from here https://xenabrowser.net/datapages/?cohort=GTEX using the GTEX data and same value (lo ...
written 15 months ago by Silvia10
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FPKM or rlog from DEseq2 for machine learning analysis
... I have RNAseq data that I have normalized using RSEM with FPKM and when I make a boxplot per sample to see the normalization plotting the log2(FPKM+0.001), the boxplot is not very good: https://ibb.co/gYgNLm On the other hand, when I plot the normalization using DEseq2 package with rlog, the boxplot ...
normalization fpkm deseq2 rna-seq written 15 months ago by Silvia10 • updated 5 days ago by Charles Warden6.1k

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